Cyflufenamid-containing sterilizing composite
A technology of cycloflufen and its composition, which is applied in the field of fungicidal compositions containing cycloflufen, to achieve the effect of expanding the control spectrum, improving the control effect, and reducing the number of times of use
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Embodiment 1
[0019] Example 1 Indoor Effect of Cyflufenamide Composition on Grape Powdery Mildew
[0020] Using the spore germination method, with the pre-test concentration as a reference, the single agent A and the single agent B original drug were diluted with a suitable solvent. Take 10 μL of the drug and spot it on a glass slide, and dry it under dust-proof conditions to make a drug film. Cut off the infected white powder grapes, brush off the white powdery mold layer with a brush, and add sterile water to make a spore suspension of appropriate concentration. Add 10 µL of the spore suspension dropwise onto the pellicle. After 16 hours of moisture cultivation at 20°C, check the germination of spores. When the germination rate of the blank control reaches about 80%, check the germination rates of all treatments. The spore germ tube length is greater than the short radius of the spore as germination, and the inhibition rate of germination is calculated according to the following formu...
Embodiment 2
[0028] Example 2 Control Effect of Cyflufenamide Composition on Cucumber Powdery Mildew
[0029] A single compound or composition is dissolved in an organic solvent to prepare a 1000 mg / L mother solution. According to the pretest of biological activity determination, a certain amount of the above mother solution was diluted with 0.1% Tween 80 to prepare the required concentration. During the test, the cucumber plants were basically at the 4-leaf stage, and the incidence of powdery mildew was investigated before the first treatment. The potted plant test of cucumber powdery mildew was carried out with the cycloflufen composition, and the corresponding single agent and blank control were set up, and the conventional constant spray was used with a hand-held sprayer, and 10 plants were sprayed with each agent, and sprayed again 7-10 days after spraying once. 10-14 days after the second spraying, investigate all the leaves of each plant, record the total number of leaves and the n...
Embodiment 3
[0046] Example 3: Field Test of Cyflufenamide Composition on Wheat Powdery Mildew
[0047] The field test of wheat powdery mildew was carried out with the composition, and a corresponding single dose and a blank control were set up, and a plot of 10m was set up for each dose. 2. A knapsack sprayer was used for conventional constant spraying, and the test was sprayed once at the booting stage and the early stage of flowering, and the investigation was carried out before the drug and 7-10 days after the second spraying. Each treatment was sampled at five points, 50 plants at each point, and each plant was investigated. For all the leaves, record and investigate the total number of leaves and the number of diseased leaves, and calculate the disease index and control effect according to the following classification method.
[0048] Leaf Grading Method:
[0049] Level 0: no disease
[0050] Level 1: The connected area of disease spots accounts for less than 1% of the entire le...
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