Power source device of electrophoresis system

Abstract

The invention relates to a power source device of an electrophoresis system. The power source device of the electrophoresis system comprises an input unit, a pre-voltage-stabilization unit, a switch transduction unit and a high-voltage transformation output unit, wherein the input unit, the pre-voltage-stabilization unit, the switch transduction unit and the high-voltage transformation output unit are connected in sequence. Grid power frequency alternating voltage is converted into a programmable error-corrected direct current power source firstly, then the direct current power source is converted into high voltage pulse signals, and then the high voltage pulse signals are converted into high voltage direct voltage and a high voltage direct current, wherein the high voltage direct voltage and the high voltage direct current are needed by the electrophoresis system. The power source device of the electrophoresis system is characterized in that the pre-voltage-stabilization unit, the switch transduction unit and the high-voltage transformation output unit and a control unit construct closed loop feedback control. The closed loop feedback control can conduct self-adaption regulations according to power source requirements of the electrophoresis system, load changes of a buffer solution and output error analysis results of the high-voltage transformation output unit, and supply the stable high voltage direct voltage and the stable high voltage direct current for the electrophoresis system in a working method of constant voltage or constant current or constant power. The power source device of the electrophoresis system can be used for an electrophoresis system which is high in requirement for power source stability and can enable a potential of hydrogen (pH) gradient of the buffer solution in the electrophoresis system to be more stable.

Description

technical field [0001] The invention relates to a power supply device, in particular to a power supply device that can be used in a large-scale liquid phase electrofocus electrophoresis system. Background technique [0002] In recent years, liquid phase electrofocus electrophoresis technology has injected new vitality into the analysis and separation of biomacromolecules. For example, in terms of protein analysis and separation, liquid phase electrofocus electrophoresis technology can minimize the loss of proteins, and can be competent for more delicate protein analysis work, as well as complete protein preparation and separation experiments. In the liquid isoelectrofocus electrophoresis system, the stability of the buffer pH gradient has always been an important factor affecting the quality of protein separation. In order to ensure the stability of the pH gradient of the buffer, people generally start from the way of adding the buffer: one of the ways is to directly inject...

AI Technical Summary

Problems solved by technology

The above two methods, the continuous filling of buffer and the uninterrupted loading of samples, will lead to unstable conductance at the entrance of the separation electric field, thus affecting the separation accuracy of electrophoresis.

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