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Salt tolerance SyGT gene derived from synechocystis, and uses thereof

A technology of cyanobacteria and salt tolerance, applied in the field of salt tolerance SyGT gene derived from cyanobacteria and its application, can solve the problems such as not achieving significant results, and achieve the effect of high application possibility and increasing salt tolerance

Inactive Publication Date: 2013-07-10
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the problem of farmland salinization, many scientists have tried to develop salt-tolerant crops through variety improvement methods such as hybridization, but no significant results have been achieved

Method used

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  • Salt tolerance SyGT gene derived from synechocystis, and uses thereof
  • Salt tolerance SyGT gene derived from synechocystis, and uses thereof
  • Salt tolerance SyGT gene derived from synechocystis, and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1: SyGT gene derived from Synechocystis sp. PCC6906

[0093] The base sequence of the SyGT gene derived from Synechocystis PCC6906 was isolated and determined after the genome of Synechocystis PCC6906 was isolated and all base sequence information was obtained using GS-FLX (Roche, USA). The SyGT gene consists of 1,149 nucleotides, encoding a 382 amino acid sequence ( figure 1 and 2 ). The amino acid sequence of the SyGT gene derived from Synechocystis PCC6906 and Synechocystis PCC6803slr0813 produced in freshwater reached 68% identity (identity), and the positive (positive) showed 83%, and its genetic relationship was the closest. In addition, genes of Cyanothece sp.PCC8801 and Cyanothece sp.ATCC51142 also showed a high genetic relationship ( image 3 ).

Embodiment 2

[0094] Example 2: Screening of SyGT Gene Transformation Vectors and Transformed Plants Derived from Synechocystis PCC6906

[0095] In order to obtain transformed plants, from PCC6906 genomic DNA, use primers 5'-gctctagaATGCAAATATTAAGCGGGTTGCAA-3' (SEQ ID NO.3, the Xb aI site is underlined) and 5'-gctctagaTTATTGGGAAAGGGGAACCA TCTT-3' (SEQ ID NO. .4, the XbaI site is underlined) After the SyGT gene was amplified, it was cut with restriction enzymes. The XbaI / XbaI site of the pHC21B vector was cut with a restriction enzyme, and the SyGT gene fragment was inserted therein, thereby preparing a nuclear transformation vector ( Figure 4 ). The plants introduced with the transformation vector are screened in a medium containing kanamycin as a selection marker. Using the primers of the SyGT gene in the screened plants, the insertion of the SyGT gene was confirmed by the PCR method. Furthermore, the expression level of the SyGT gene in each transformed plant was analyzed by real-time...

Embodiment 3

[0096] Example 3: Preparation of tobacco transformed with SyGT gene derived from Synechocystis sp. PCC6906

[0097] Reserve and sterilize T0 generation seeds from tobacco transformed plants introduced with SyGT gene derived from Synechocystis sp. , thus preserving the T1 generation seeds. Plants were obtained from the retained seeds, and genomic DNA was isolated, and the introduction of SyGT was confirmed by PCR and Southern blot hybridization analysis, and total RNA was isolated, and the expression level of the introduced genes was confirmed by real-time quantitative PCR. Such as Figure 5 As shown in A, among the 21 kinds of SyGT gene-transformed tobacco plants, 14 kinds of transformants were confirmed to have introduced the gene, and in these transformed plants, it was confirmed that 4 kinds had inserted a copy of the gene ( Figure 5 B).

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Abstract

The present invention relates to a method for increasing the salt tolerance of a plant, to a plant having increased salt tolerance prepared by the method, and to a seed of the plant, wherein the method comprises over-expressing SyGT by transforming a plant cell with a recombinant plant expression vector including a gene encoding Synechocystis PCC 6906-derived SyGT protein and a SyGT gene.

Description

technical field [0001] The present invention relates to a Synechocystis glucosyl transferase (SyGT) gene derived from cyanobacteria, a method for overexpressing the gene in a plant to increase the salt tolerance of the plant, and the The plant body and its seeds with increased salt tolerance prepared by the method. Background technique [0002] Algae containing cyanobacteria have been exploited as efficient genetic resources, bioenergy (red algae ethanol and seaweed biodiesel) and efficient biomaterials (red algae slurry). Recently, the applicability of seaweed has been broadened through the application of seaweed bioengineering, and research on seaweed is underway. For example, new product development using molecular breeding, bioreactors for the production of effective proteins or effective substances that are important for medicine or industry. [0003] Recently, it has been reported that many genes found in cyanobacteria have been successfully applied to crops. Genes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C07K14/195C12N15/31C12N15/82
CPCC07K14/195C12N9/1051C12N15/8273C12Y204/0108C07K14/405C12Q1/6895C12Y204/01Y02A40/146C12N15/8271
Inventor 刘长烈金锡源金宗炫闵成兰郑元重安明淑朴英敏吳明珍朴祉泫
Owner KOREA RES INST OF BIOSCI & BIOTECH
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