Preparation method of efficient composite micro-ecologic premix for animals

A composite microecological and premix technology, which is applied in the preparation of veterinary high-efficiency composite microecological premix and the field of microecological premix preparation, can solve the problem that high temperature processing and storage process are unstable and affect the microecological premix. In order to improve animal production performance, improve feed utilization, and reduce body stress

Inactive Publication Date: 2013-07-17
SICHUAN HUANYA BIOTECH
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the veterinary microecological premix products on the domestic market mainly have the following deficiencies: (1) The content of live bacteria is low. 7 1 / g, the enzymes and metabolites produced by the bacteria are not enough to affect the host, and it is difficult to meet the needs of treatment; (2) The stability to gastric acid and bile is poor, so that only a few strains are still active after entering the intestinal tract, and cannot play a role. The number of viable bacteria required; (3) poor proliferation rate in the body; (4) not resistant to high copper, unstable during high temperature processing and storage, and short shelf life; (5) few compound premixes, only suitable for a specific animal
This affects the application effect of the probiotic premix on animals and limits the scope of application of the probiotic premix

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method for a high-efficiency compound microecological premix for animals, comprising the following steps:

[0038] 1) Strain optimization screening

[0039] The strains of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae were respectively acclimated to acid resistance, bile salt resistance, temperature resistance and high copper resistance;

[0040] The acid-resistant acclimatization refers to the selection of acid-resistant bacterial strains under the condition of pH 3.2-3.6;

[0041] The bile-salt-resistant acclimatization refers to: after adding bile-salts to the culture medium, the selected acid-resistant bacterial strains are acclimatized;

[0042] The temperature-resistant acclimation refers to: inoculating the bacterial cultures acclimatized to bile salts into LB and TB liquid medium, then heating in a water bath at 40-60°C, and then continuing to culture the culture at 37°C;

[0043] The high-copper-resistant...

Embodiment 2

[0062] A preparation method for a high-efficiency compound microecological premix for animals, comprising the following steps:

[0063] 1) Strain optimization screening

[0064] The strains of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae were respectively acclimated to acid resistance, bile salt resistance, temperature resistance and high copper resistance;

[0065] The acid-resistant acclimatization refers to the selection of acid-resistant bacterial strains under the condition of pH 3.2-3.6;

[0066] The bile-salt-resistant acclimatization refers to: after adding bile-salts to the culture medium, the selected acid-resistant bacterial strains are acclimatized;

[0067] The temperature-resistant acclimation refers to: inoculating the bacterial cultures acclimatized to bile salts into LB and TB liquid medium, then heating in a water bath at 40-60°C, and then continuing to culture the culture at 37°C;

[0068] The high-copper-resistant...

Embodiment 3

[0087] A preparation method for a high-efficiency compound microecological premix for animals, comprising the following steps:

[0088] 1) Strain optimization screening

[0089] The strains of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae were respectively acclimated to acid resistance, bile salt resistance, temperature resistance and high copper resistance;

[0090] The acid-resistant acclimatization refers to the selection of acid-resistant bacterial strains under the condition of pH 3.2-3.6;

[0091] The bile-salt-resistant acclimatization refers to: after adding bile-salts to the culture medium, the selected acid-resistant bacterial strains are acclimatized;

[0092] The temperature-resistant acclimation refers to: inoculating the bacterial cultures acclimatized to bile salts into LB and TB liquid medium, then heating in a water bath at 40-60°C, and then continuing to culture the culture at 37°C;

[0093] The high-copper-resistant...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of an efficient composite micro-ecologic premix for animals, and belongs to the technical field of micro-ecologic preparation feed additives. The preparation method comprises the following steps: a step of respective optimal screening of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae, a step of bacterial strain rejuvenation, a step of shaking culture, a step of culturing in a 30L fermentation cylinder, a step of thallus spray drying, and a step of preparing composite bacterial powder. The efficient composite micro-ecologic premix prepared through the method has the characteristics of excellent acid, salt, thermal and bile resistances, high content of live bacteria, high-copper tolerance, and good stability under feed granulation processing and normal temperature preservation conditions, the preparation method of the premix is simple and has a low cost, and the premix product produced through the method has a stable and reliable quality, and has a good market prospect.

Description

technical field [0001] The invention relates to a preparation method of a microecological premix, more specifically to a preparation method of a high-efficiency compound microecological premix for animals, and belongs to the technical field of probiotic feed additives. Background technique [0002] Veterinary micro-ecological premix is ​​a natural, green and safe feed additive. Due to its advantages of non-toxic, harmless, no residue and no pollution, it can prevent and treat diseases, improve feed utilization, and improve animal health. Production performance and other effects, and can overcome the bacterial flora imbalance, superinfection and drug resistance caused by antibiotics, and have been widely studied and applied in the case of increasingly prominent animal-derived food safety problems. [0003] At present, the veterinary microecological premix products on the domestic market mainly have the following deficiencies: (1) The content of live bacteria is low. 7 1 / g, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23K1/18A23K1/16C12N1/20C12N1/18C12N1/16C12R1/10C12R1/125C12R1/72C12R1/865
Inventor 赵志毅胥勇温亮
Owner SICHUAN HUANYA BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap