Establishment method and application of mass spectrum database of prohibited colorant in foods
A technology for establishing methods and colorants, applied in special data processing applications, electrical digital data processing, measuring devices, etc., can solve problems such as inability to handle, inability to detect, and identification of detection institutions, and achieve the effect of improving product quality
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] In this embodiment, 102 colorants shown in Table 1 were selected for sorting and analysis, and a Q-TOF mass spectrum database was established according to the method of the present invention. Among them, there are 16 kinds of edible pigments and 86 kinds of industrial dyes prohibited in food, including acid dyes, basic dyes, direct dyes, disperse dyes, reactive dyes, mordant dyes, etc.
[0038] Enter the collected information such as the compound name, CAS number, neutral molecular formula, and structural formula of the colorant shown in Table 1 into the edit compound information entry in the Personal Compound Database and Libarary Mangager (PCDL) software; and then , prepare the collected standard stock solution and working solution of the library building compound respectively, adjust the chromatographic conditions and mass spectrometry conditions, and collect the first-order spectrum data in MS mode for the working solution of the standard; the instruments, equipment ...
Embodiment 2
[0061] After the colorant mass spectrum database described in Example 1 was established, it was found that the exact molecular weights of some colorants with similar properties were very close. In order to distinguish such compounds, this embodiment chromatographically separates such compounds by using chromatographic columns and adjusting the ratio and type of mobile phase. First, the colorants are grouped according to their molecular weight, and are summarized into eight groups of compounds, see Table 3.
[0062] The eight groups of compound analysis mass spectrometry conditions are as follows:
[0063] Jet stream ESI source; nozzle voltage is 1000V, capillary voltage is 3500V, collision voltage is 130V, cone voltage is 65V; drying gas temperature is 350°C; drying gas flow rate is 5L / min; atomizer pressure is 45psi; sheath gas The temperature is 350°C; the flow rate of the sheath gas is 11L / min; the mass spectrometry acquisition mode is MS mode, and the mass spectrometry ac...
Embodiment 3
[0085] Qualitative and quantitative detection of Congo red in beef in embodiment 3
[0086] The identification method of illegally added Congo red in food discussed in this example is expected to provide a good solution for the identification and quantification of illegal additives, and provide a guarantee for food quality control and safety evaluation.
[0087] Instruments and materials:
[0088] 1200RRLC high performance liquid chromatograph connected in series with 6530 quadrupole-time-of-flight mass spectrometer, MassHunter chromatographic workstation and PCDL software self-built colorant mass spectrogram database (Agilent, USA); XW-80A vortex mixer (Huxi, Qingpu, Shanghai) Instrument Factory); Avanti J-E centrifuge (Beckman Coulter, USA); 5418R centrifuge (Eppendorf China Co., Ltd.); XS-205DU electronic analytical balance (Mettler-Toledo, USA); FW100 high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.).
[0089] Beef samples are commercial samples.
...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More