Activated probiotic skin cream and preparation method thereof
A technology of active probiotics and skin cream, which is applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of easy decline and inactivation of probiotics, unapplied probiotics, and low stability of live bacteria. Achieving the effect of enriching varieties, efficient biological protection, and balancing the pH of the skin surface
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Embodiment 1
[0027] In the first step, Streptococcus thermophilus (Streptococcsus thermophilus) was cultured at a high density in the medium in the microbial batch culture fermentor at 40°C, and the number of viable bacteria in the culture solution reached 6.5×10 9 CFU / mL, and then centrifuge at 15000rpm for 5 minutes to obtain bacterial sludge. The number of viable bacteria in bacterial sludge is 1×10 11 CFU / g;
[0028] In the second step, mix glycerin and trehalose at a mass ratio of 80:20. After the trehalose is completely dissolved in a water bath at 90°C, heat it at 95°C, keep it for 15 minutes, and then sterilize it, then cool it to 4°C in a sealed manner;
[0029] In the third step, the bacterial sludge obtained in the first step is mixed with the mixed liquid obtained in the second step at a mass ratio of 1:10, and the mixture is ground and stirred to form a bacterial slurry;
[0030] The fourth step is to filter the bacterial slurry with a 200-mesh sieve, and the filtrate is a probiotic b...
Embodiment 2
[0034] In the first step, Lactobacillus casei subsp casei (Lactobacillus casei subsp casei) is cultured in a high-density medium at 37°C in a microbial batch culture fermentation tank, and the number of viable cells in the culture solution is 1.2×10 9 CFU / g, then centrifuged at 10000rpm for 10 minutes to obtain bacterial sludge, the number of viable bacterial sludge is 3×10 10 CFU / g;
[0035] In the second step, glycerin and trehalose are mixed in a mass ratio of 85:15. After the trehalose is completely dissolved in a water bath at 85°C, heat it at 90°C, keep it for 20 minutes, and then sterilize it, then cool it in an airtight manner and refrigerate at 4°C;
[0036] In the third step, the bacterial sludge obtained in the first step is mixed with the mixed liquid obtained in the second step at a mass ratio of 1:100, and the mixture is ground and stirred to form a bacterial slurry;
[0037] The fourth step is to filter the bacterial slurry with a 300-mesh sieve, the filtrate is a probi...
Embodiment 3
[0041] In the first step, Streptococcus thermophilus (Streptococcsus thermophilus) was cultured at a high density in the medium in the microbial batch culture fermentor at 40°C, and the number of viable bacteria in the culture solution reached 6.5×10 9 CFU / g, then centrifuged at 15000rpm for 5 minutes to obtain bacterial sludge, the number of viable bacterial sludge is 1×10 11 CFU / g;
[0042] In the second step, Lactobacillus casei subsp casei is cultured in high density at 37°C in the medium in the microbial batch culture fermentation tank, and the number of viable cells in the culture solution reaches 1.5×10 10 CFU / g, then centrifuged at 15000rpm for 10 minutes to obtain bacterial sludge, the number of viable bacterial sludge is 6×10 11 CFU / g;
[0043] The third step is to mix glycerol and trehalose according to the mass ratio of glycerol and trehalose at a mass ratio of 90:10. After the trehalose is completely dissolved in a water bath at 90°C, heat it at 95°C, keep it for 20 minu...
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