Method for mycelium pellet to form mixed mycelium pellet by adsorbing photosynthetic bacteria
A technology for photosynthetic bacteria and mycelium balls, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems that have not been reported yet, and achieve improved treatment effect, good adsorption effect, and culture. short time effect
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Embodiment 1
[0030] 1. Preparation of spore suspension
[0031] On the aseptic operating table, gently scrape the spores of Penicillium DH-1 growing on the solid medium on the slope with an inoculation loop to make the spores fall off. Rinse the solid slope repeatedly with 10 mL of sterile saline until the spores are suspended in the saline. Use a pipette to transfer the rinsed physiological saline containing Penicillium DH-1 spores to a 100 ml Erlenmeyer flask, and add sterile deionized water so that each ml of deionized water contains about 108 spores. Then place it in an air bath shaker and shake for 1 hour, so that the spores are uniformly dispersed, and the spore suspension is obtained.
[0032] 2. Preparation of mycelium balls
[0033] 1. Preparation of Mycelial Ball Liquid Medium
[0034] Weigh 10 g of glucose, 5 g of peptone, 1 g of KH2PO4, and 0.5 g of MgSO4 7H2O into 1000 ml of sterile deionized water, adjust the pH to 7.0 with 10% NaOH and 10% HCl, and stir well. It is mycel...
Embodiment 2
[0051] 1. Preparation of spore suspension
[0052] On the sterile operating table, gently scrape the mold DH-1 spores growing on the slant solid medium with an inoculation loop to make the spores fall off. Rinse the solid slant repeatedly with 10 ml of sterile saline until the spores are suspended in the saline. Use a pipette to transfer the rinsed physiological saline containing DH-1 spores to a 100 ml Erlenmeyer flask, and add sterile deionized water so that each ml of deionized water contains about 108 spores. Then place it in an air bath shaker and shake for 1 hour, so that the spores are uniformly dispersed, and the spore suspension is obtained.
[0053] 2. Preparation of mycelium balls
[0054] 1. Preparation of Mycelial Ball Liquid Medium
[0055] Weigh 5 g of soluble starch, 0.5 g of yeast extract, 0.5 g of KH2PO4, and 1 g of MgSO4 7H2O into 1000 ml of sterile deionized water, adjust the pH to 7.5 with 10% NaOH and 10% HCl, and stir well , which is the mycelium liq...
Embodiment 3
[0072] 1. Preparation of spore suspension
[0073] On the aseptic operating table, gently scrape the spores of Penicillium DH-1 growing on the solid medium on the slope with an inoculation loop to make the spores fall off. Rinse the solid slant repeatedly with 10 ml of sterile saline until the spores are suspended in the saline. Use a pipette to transfer the rinsed physiological saline containing DH-1 spores to a 100 ml Erlenmeyer flask, and add sterile deionized water so that each ml of deionized water contains about 108 spores. Then place it in an air bath shaker and shake for 1 hour, so that the spores are uniformly dispersed, and the spore suspension is obtained.
[0074] 2. Preparation of mycelium balls
[0075] 1. Preparation of Mycelial Ball Liquid Medium
[0076] Weigh 8 g of sucrose, 1 g of beef extract, 0.3 g of KH2PO4, and 0.5 g of MgSO4 7H2O into 1000 ml of sterile deionized water, adjust the pH to 8.0 with 10% NaOH and 10% HCl, and stir well. It is mycelium li...
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