Method and device for regulating tower plate type perfusion bioreactor

A technology for bioreactors and regulating devices, which is applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical cleaning device, etc. Fluid flow rate, fluid shear force, lack of structural parameter optimization methods, etc.

Active Publication Date: 2013-08-28
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0007] (2) The existing tray-type perfusion bioreactor cannot adjust its key structural parameters, and it is difficult to obtain the fluid shear force generated by a consistent fluid flow rate
[0008] (3) The existing tray-type perfusion bioreactor lacks structural parameter optimization methods for scale-up cultivation, and it is difficult to obtain optimal fluid shear force for cell culture between each layer of trays

Method used

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  • Method and device for regulating tower plate type perfusion bioreactor
  • Method and device for regulating tower plate type perfusion bioreactor
  • Method and device for regulating tower plate type perfusion bioreactor

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Embodiment Construction

[0038]The accompanying drawings are for illustrative purposes only, and should not be construed as limitations on this patent; in order to better illustrate this embodiment, certain components in the accompanying drawings will be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art It is understandable that some well-known structures and descriptions thereof may be omitted in the drawings.

[0039] Such as image 3 Shown, a kind of regulation method of tray type perfusion bioreactor, comprises the following steps:

[0040] S1. Adjust the regulating valves on the trays of each layer so that each tray has the same fluid inlet structure parameters; place the trays vertically in a culture tank filled with culture fluid, and the cells planted on the trays are circulating Cultivate in the culture medium of flow perfusion;

[0041] S2. Cultivate cells under the same fluid inlet structural parameters; because the structural ...

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Abstract

The invention relates to the technical field of bioreactors in medical instruments, and in particular relates to a method and a device for regulating a tower plate type perfusion bioreactor. The method for regulating the tower plate type perfusion bioreactor comprises the following steps of: (1), regulating a regulating valve on each layer of tower plate; (2), cultivating cells under same fluid inlet structure parameters; (3), determining a tower plate layer number N with an optimum cultivation effect; (4), computing the flowing speed M of a fluid on the surface of an N-th layer of tower plate; (5), computing structure parameters A(i) of a fluid inlet between adjacent layers of tower plates; (6), regulating the regulating valve on each layer of tower plate; and (7), continuing cultivating the cells on the surface of each layer of regulated tower plate. According to the method and the device, the structure parameters of an inflow port in each layer of tower plate in the perfusion bioreactor can be regulated, so that the fluid shear stress between the adjacent layers of tower plates can be regulated according to a geometric structure, an optimization way is provided for distribution of the fluid shear stresses in each layer of tower plate, and the fluid shear stresses of all layers of tower plates can be optimized.

Description

technical field [0001] The invention relates to the technical field of bioreactors in medical devices, and more specifically, to a method for adjusting a tray-type perfusion bioreactor and a device for using the same. Background technique [0002] The structure of the existing bioreactor is as follows figure 1 As shown, its function is to create an environment similar to that in the body in vitro to accelerate the cultivation of cells and the formation of tissues and even organs. It can be widely used in patients with organ defects to replace, construct, maintain or enhance their tissue functions, as well as biopharmaceuticals, etc. field. The principle is to use engineering technology to construct a set of devices in vitro based on the physiological, physical, and chemical characteristics of the microenvironment in which cells grow in vivo, so that it can provide a microenvironment similar to that in vivo, so as to facilitate the growth of cells in this in vitro microenvir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q3/00C12M1/36
Inventor 罗语溪高玉宝龚逸鸿蒋庆
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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