Micro-needle enzyme-free glucose sensor electrode and preparation method thereof

A glucose sensor and microneedle technology, applied in the field of biosensors, can solve the problems of low electroactivity of stainless steel, small diameter of acupuncture needle electrodes, small surface area, etc., and achieve the effect of large specific surface area and improved electrocatalytic performance

Inactive Publication Date: 2013-08-28
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stainless steel acupuncture needle electrodes are used to construct implantable glucose sensors, which have the advantages of small area, easy implantation and portability, and can meet the requirements of real-time online monitoring blood glucose meters for sensor miniaturization. However, the surface area of ​​bare stainless steel needle electrodes is very small. , at the same time, the electrical activity of stainless steel itself is also very small, so the surface modification of stainless steel needle electrodes

Method used

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  • Micro-needle enzyme-free glucose sensor electrode and preparation method thereof
  • Micro-needle enzyme-free glucose sensor electrode and preparation method thereof
  • Micro-needle enzyme-free glucose sensor electrode and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Soak the stainless steel needle electrode in acetone, and ultrasonically clean it to remove the surface oil, then wash it repeatedly with ethanol, ultrasonically, dry it in the air, place it in a PBS solution with a pH value of 7.0, and activate it by electrochemical cyclic voltammetry scanning for 10 cycles . Then, the graphene oxide was dispersed in a chitosan solution with a mass concentration of 0.5%, and after magnetic stirring at room temperature for 4 hours, polyvinyl alcohol, chloroplatinic acid and lead acetate were added to the above solution, and the concentration of chloroplatinic acid in the mixed solution The concentration of lead acetate is 25mg / ml, the concentration of lead acetate is 50mg / ml, the concentration of polyvinyl alcohol is 8%, and the concentration of graphene oxide is 7mg / ml. Finally, the above solution was injected into a syringe with a needle (No. 10, inner diameter 0.6 mm, length 15 mm, and the tip was ground flat), and the syringe was fi...

Embodiment 2

[0030] The operation process and experimental conditions of Example 2 are the same as those of Example 1, except that the interval time of ultrasonic vibration is changed to 3 minutes.

[0031] The prepared electrode was used as the working electrode, the Pt wire electrode was used as the auxiliary electrode, and the saturated calomel electrode was used as the reference electrode. Into 30ml of blank PBS, 30μL of 1.5mol / L glucose solution was added dropwise successively, and after each dropwise addition, differential pulse voltammetry (DPV) measurement was carried out. figure 2 The results show that the sensitivity of the prepared sensor is 5.62μA*(cm -2 mM -1 ), the linear monitoring range is 2-24mM, and the detection limit is 1.6μM. Compared with the sensor disclosed in the patent ZL201010275952.6, it has wide linear range, low detection limit and high sensitivity.

Embodiment 3

[0033] The operating process and experimental conditions of Example 3 are the same as in Example 1, except that the concentration of graphene oxide is changed to 10 mg / ml.

[0034] The prepared electrode was used as the working electrode, the Pt wire electrode was used as the auxiliary electrode, and the saturated calomel electrode was used as the reference electrode. Into 30ml of blank PBS, 30μL of 1.5mol / L glucose solution was added dropwise successively, and after each dropwise addition, differential pulse voltammetry (DPV) measurement was carried out. image 3 The results show that the sensitivity of the prepared sensor is 6.24μA*(cm -2 mM -1), the linear monitoring range is 2-22mM, and the detection limit is 1.42μM. Compared with the sensor disclosed in the patent ZL201010275952.6, it has wide linear range, low detection limit and high sensitivity.

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Abstract

The invention discloses a micro-needle enzyme-free glucose sensor electrode and a preparation method thereof, and is characterized in that the electrode is composed of graphene nanofibers loaded Pt-Pb nanoflower. The preparation process of the electrode comprises: preparing graphene oxide/chitosan/metal salt nanofibers on a surface of a micro-needle electrode through an electrospinning method, and then preparing the graphene nanofibers loaded Pt-Pb nanoflower micro-needle electrode through an ultrasonic intermittent-vibration constant-current electrodeposition method. The invention is advantageous in that the enzyme-free glucose sensor electrode can be constructed on the surface of the micro-needle electrode; the electrode has a good electro-catalytic oxidation property for the glucose; the constructed micro-needle enzyme-free glucose sensor has a wide linear range for the glucose and is high in response sensitivity, low in detection limit, high in stability, and good in application prospects in an aspect of online dynamic blood glucose testing in real time.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a microneedle-type enzyme-free glucose sensor electrode and a preparation method thereof. Background technique [0002] Continuous blood glucose monitoring has important clinical significance for the treatment of diabetic patients and the screening of early childhood diabetes. However, the current main methods of blood glucose detection include invasive blood glucose detectors and continuous blood glucose monitoring systems. Invasive blood glucose detectors are frequently operated and cannot continuously monitor blood glucose concentrations; , requires the aid of a syringe when implanted into the wearer's body. In order to meet the needs of real-time on-line monitoring blood glucose meters for the precision and miniaturization of implanted glucose sensors, as well as the requirements for sensor stability, repeatability and selectivity, the construction of micro-needle glucose sensors h...

Claims

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Application Information

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IPC IPC(8): G01N27/30
Inventor 郭美卿王鹤峰陈静史振东
Owner TAIYUAN UNIV OF TECH
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