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Marking primer and method for identifying channa argus and channa maculate as well as F1-generation channa argus*channa maculate and channa maculate*channa argus hybridized by channa argus and channa maculate

A technology for labeling primers and snakeheads, which is applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problem of being unable to distinguish between two hybrids, and achieve short cycle time and simple methods Effect

Inactive Publication Date: 2013-09-04
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, the nuclear genetic material source of the hybrid snakeheads of the orthogonal combination and the hybrid snakeheads of the reverse cross combination is the same, and the molecular markers of nuclear DNA cannot be used to distinguish the two hybrids

Method used

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  • Marking primer and method for identifying channa argus and channa maculate as well as F1-generation channa argus*channa maculate and channa maculate*channa argus hybridized by channa argus and channa maculate
  • Marking primer and method for identifying channa argus and channa maculate as well as F1-generation channa argus*channa maculate and channa maculate*channa argus hybridized by channa argus and channa maculate

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Embodiment 1

[0025] The present invention provides a marker primer for identification of snakeheads, snakeheads and their hybrid F1 generation of snakeheads and snakeheads based on mitochondrial DNA sequences. The left end primer sequence BY14F is: 5'-CCCGTCACCCCTCACCTAA-3', and the right end primer sequence BY14R is: 5'-GCGTGGGGCTACCTGTTC-3'.

Embodiment 2

[0027] The method provided by the present invention for identifying Snakehead and Snakehead using the marker primers for identifying Snakehead, Snakehead and its hybrid F1 generation Snakehead and Snakehead based on the mitochondrial DNA sequence in Example 1, using the markers in Example 1 Primers, to amplify Snakehead, Snakehead, Snakehead and Snakehead, and Snakehead and Snakehead have PCR-specific bands at 201bp, while Snakehead and Snakehead have no bands, thus identifying Snakehead and black-spotted snakehead.

[0028] The labeled primers in the present invention are obtained by the following methods:

[0029] (1) Take 30 fishes of black snakehead, spotted snakehead, black snakehead (snakehead x black snakehead), and black snakehead (snakehead x black snakehead), cut their fin rays, and store them in 95% ethanol at -20°C for later use. The whole genome DNA of the four fish species was extracted by conventional methods in the field, and stored at -20°C for future use.

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Abstract

The invention discloses a marking primer for identifying channa argus and channa maculate as well as F1-generation channa argus*channa maculate and channa maculate*channa argus hybridized by the channa argus and the channa maculate on the basis of a mitochondrial DNA (deoxyribonucleic acid) sequence. The marking primer is BY14, and the marking primer BY14 consists of a left-end primer sequence BY14F and a right-end primer sequence BY14R, wherein the left-end primer sequence BY14F is 5'-CCCGTCACCCTCACCTAA-3', and the right-end primer sequence BY14R is 5'-GCGTGGGGCTACCTGTTC-3'. The invention further discloses a method for identifying the channa argus and the channa maculate as well as the F1-generation channa argus*channa maculate and channa maculate*channa argus hybridized by the channa argus and the channa maculate by using the marking primer. The marking primer can be used for rapidly identifying the channa argus and channa maculate as well as the F1-generation channa argus*channa maculate and channa maculate*channa argus hybridized by the channa argus and the channa maculate.

Description

technical field [0001] The invention specifically relates to a marker primer and a method for identifying snakeheads, snakeheads and hybrid F1 generation snakeheads and snakeheads. Background technique [0002] Snakehead ( Channa Argus ) and Channa ( Channa maculata ) belongs to Perciformes (Perciformes), Channidae suborder (Channidae), Channidae (Channidae), Channa genus ( Channa ). The black snakehead is mainly distributed in the Yangtze River and the river systems north of the Yangtze River, while the spotted snakehead is mainly distributed in the Pearl River and Hainan water systems. These two types of fish have always been favorite objects in the food tradition, so the market demand is very high, which has given birth to the aquaculture industry of the snakehead fish. In the past 10 years, the aquaculture area of ​​Channa has been expanding, and the aquaculture output has been rising steadily. By 2011, the total aquaculture production of snakeheads in China had r...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 朱新平张新铖陈昆慈赵建李伟罗青
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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