Electrical signal calibration method for solid-state nanopore DNA sequencing
A DNA sequencing and calibration method technology, applied in the fields of genetics and molecular biology, can solve the problems of exceeding the range of the analog-to-digital converter and insufficient resolution of the detection waveform, and achieve the effect of improving the resolution
Active Publication Date: 2015-03-04
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology
If the same magnification is used, for bases with small characteristic currents, there may be a problem of insufficient detection waveform resolution; for bases with large characteristic currents, there may be problems beyond the range of the analog-to-digital converter
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[0025] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0026] The definitions of technical terms that need to be used in the present invention are as follows:
[0027] (1) Full-scale data: Considering the fluctuation of the base signal, the full-scale data is defined as 75% to 85% of the actual range of the analog-to-digital converter. This value can be adjusted according to the actual situation;
[0028] (2) Optimum gain value: After collecting voltage data based on bases collected under the base gain value, and extracting the statistical feature data of its time series, for the channel data that is not in full range, after considering its signal flu...
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The invention discloses an electrical signal calibration method for solid-state nanopore DNA sequencing. The method comprises: S1, setting a gain initial basic value of each amplifier in a program control amplification circuit; S2, acquiring in parallel by an analog-to-digital converter in each channel to obtain base acquisition voltage data corresponding to each gain initial basic value; S3, successively determining whether the base acquisition voltage data of the each channel is less than full-scale data, if yes, going into S5, and if no, going into S4; S4, discarding the base acquisition voltage data of a full-scale channel; S5, selecting the base acquisition voltage data having a largest resolution, and calculating an optimum gain value according to the base acquisition voltage data having the largest resolution; S6, determining whether a gain initial basic value in a current channel is equal to the optimum gain value, if yes, stopping, and if no, going into S7; and S7, correcting the gain initial basic value in the current channel to the optimum gain value. The electrical signal calibration method aims at different base characteristic current during a nucleic acid sequencing process, by setting different gains to improve an identification resolution.
Description
technical field [0001] The invention belongs to the fields of genetics and molecular biology, and more specifically relates to an electrical signal calibration method for solid-state nanopore DNA sequencing. Background technique [0002] Nanopore DNA sequencing technology is an emerging rapid non-destructive detection technology. Due to its electronic detection method and convenient sample preparation, nanopore DNA sequencing has great potential in the field of cheap sequencing, and is by far the most promising technology to realize the whole gene "$1,000" DNA sequencing project. [0003] Nanopore nucleic acid sequencing mostly adopts the method of electrophoresis, and bases will cause changes in current when they pass through nanoscale pores driven by an external constant electric field. Due to the differences in size and molecular structure of different bases, characteristic currents with different amplitudes and pulse widths will be generated, and the order in which the ...
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IPC IPC(8): G01N27/447
Inventor 张浩曹忠升瞿安连蔡子龙
Owner HUAZHONG UNIV OF SCI & TECH