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Device and method for detecting gas by utilizing honeybees

A technology for detecting gas and gas to be measured, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high demand for computer and hardware resources, interference of test results, and easy occurrence of missed detection, so as to improve the accuracy of online detection, The effect of saving the gas to be tested and improving the detection accuracy

Inactive Publication Date: 2013-09-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The inside of the device is a full-light environment, and it is not conducive for bees to keep calm in a full-light environment. In an environment that does not contain training gas, beak and tongue behavior may also occur, which not only interferes with the test results, but also It is also not conducive to the survival of bees;
[0006] (2) The camera captures the image of the entire gas detection chamber or the head of the bee, and then uses image processing technology to extract the beak and tongue action of the bee in the image. Compared with the surrounding environment, the bee's beak and tongue The volume and area of ​​the part are very small, the identification is difficult, and errors such as missed detection are prone to occur. Not only is the operation complicated, but the demand for computer and hardware resources is also high;
[0007] (3) Moreover, since the antennae of the bees are constantly moving during the detection process, the monitoring of the tongue-throwing behavior will be interfered when the image is extracted, and the above-mentioned patent documents do not propose an effective solution to this;
[0008] (4) When the substance to be detected contains two or more marker gases at the same time, the above patent literature does not propose a solution for simultaneously detecting multiple marker gases to determine the substance to be detected

Method used

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  • Device and method for detecting gas by utilizing honeybees
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  • Device and method for detecting gas by utilizing honeybees

Examples

Experimental program
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Embodiment 1

[0049] Such as Figure 4 As shown, a device for detecting gas by using bees in the present invention includes a housing 1, and there are four spaces in the housing 1, which are respectively: a gas feeding chamber 20, a dark detection chamber 30, an image acquisition chamber 50 and a gas filter chamber 40 .

[0050] Such as Figure 6 As shown, a feed main pipe 25 is arranged in the gas feed chamber 20, and one end of the feed pipe 25 protrudes from the gas feed chamber 20, and a gas delivery pump 21 is installed at the gas inlet for transferring the The gas to be tested is pumped into the device; the main feed pipe 25 is also provided with a gas injection port 22 for injecting the collected gas to be tested into the device.

[0051] Depend on Figure 4 , Figure 6 It can be seen that the other end of the feeding main pipe 25 is connected with two feeding branch pipes 23, and each feeding branch pipe 23 is connected with four feeding conduits 24 respectively, and the feeding...

Embodiment 2

[0057] A kind of method that utilizes honeybee to detect gas of the present invention is to pass such as Figure 1 to Figure 6 The device shown is realized, specifically including:

[0058] (1) Capture bees: capture worker bees who go out to collect honey near the entrance and exit of the hive, usually in the morning or afternoon, when mature worker bees are easy to capture;

[0059] (2) Fix the bees: place the bees in a refrigerated environment at about 4°C for about 5 minutes to prevent the bees from moving; then place the bees in a fixed small tube with a gap, cut the tape of appropriate width to fix the body of the bees , only the head of the bee is higher than the wall of the fixed small tube; then use a thinner tape to bind the joint of the bee's head and body to fix the head of the bee; after fixing, only the antennae, beak and tongue can move; after fixing is completed , fed with sucrose solution, placed overnight in a humid and dark room temperature environment, so t...

Embodiment 3

[0071] Use orange oil volatile gas (the preparation method is the same as lavender oil volatile gas) as training gas, and use 100mL and 500mL orange oil volatile gas to float to 100×100×100mm respectively. 3 The gas obtained in the cube glass space is the gas to be tested, and the gas to be tested is detected by the same device and method as in Example 2.

[0072] After monitoring, all eight testing bees had the behavior of stretching their beaks and tongues, indicating that the volatile gas of orange oil in the gas to be tested was detected.

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Abstract

The invention discloses a device and a method for detecting gas by utilizing honeybees. The device comprises a darkroom which is internally provided with a plurality of honeybee fixing seats, feeding guide pipes for conveying gas to be detected into the darkroom, and an image collection unit for monitoring a honeybee state, wherein a plurality of the feeding guide pipes are arranged and correspond to the honeybee fixing seats one by one; and the image collection unit comprises detection light sources which correspond to the honeybee fixing seats one by one, and image collectors arranged above the honeybee fixing seats. The method comprises the following steps of: carrying out Pavlov conditioned reflex training on the honeybees; fixing the trained honeybees in the honeybee fixing seats and conveying the gas to be conveyed in front of tentacles of the honeybees; and collecting and detecting honeybee mouth images of a light-emitting opening of the detection light source by the image collectors, and judging the gas to be detected according to the collected images. Compared with the prior art, the device disclosed by the invention is high in detection precision and accuracy, and two or more than two types of gas components in the gas to be detected can be detected.

Description

technical field [0001] The invention relates to the technical field of gas detection, in particular to a device and method for detecting gas by using bees. Background technique [0002] In most cases, gases are detected in the laboratory using chemical methods or biochemical sensors. In special cases, the rapid detection of certain specific gases (such as gases emitted from drugs or explosives) is carried out by using strictly trained animals, such as the use of drug detection dogs in drug inspections; however, the training of these animals is expensive and difficult to obtain. use. [0003] Bees are animals with a strong sense of smell, and their antennae are covered with various receptors, which are extremely sensitive to specific gases. Moreover, honeybees have strong learning and memory abilities, and the Pavlovian reflex for training gases can be constructed by using the correlation learning of bees for specific gases. Use Pavlov's conditioned reflex principle to tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 尚春刚李光王雅圣
Owner ZHEJIANG UNIV
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