Method for making agaricus blazei fungus strain, ie original seed of wheat grain, by using white loam as filter layer

Abstract

The invention provides a method for preparing agaricus blazei murill wheat grain strains, namely original wheat grain seeds, by utilizing white loam as a filter layer. The method comprises the processing steps as follows: firstly, producing mother strains; secondly, producing original wheat grain seeds; and thirdly, producing wheat grain cultivated species. The method is characterized in that the processing step for preparing the original wheat grain seeds or the wheat grain cultivated species comprises the steps as follows: material preparation is performed and includes that a wheat grain culture medium is processed and prepared for reservation, and sufficient natural white loam is taken for reservation; water content of the white loam is regulated in a range from 24% to 27%, and pH value is regulated in a range from 7.3 to 7.7; the wheat grain culture medium is bottled according to a conventional method, then the white loam is placed into a bottle, the placed white loam is located above the wheat grain culture medium and has a 4-5 cm distance away from a bottle mouth, and the thickness of the white loam ranges from 1.5 cm to 2 cm; inoculation is performed after sterilization treatment; and then the culture bottle is placed in a culture room for original seed or cultivated specie cultivation according to the common method. The method has an effect of filtering infectious microbes, infectious microbe contamination during a preparation process can be avoided to the greatest extent, and the rate of finished products can reach 99%-100%; and moreover, raw materials of the filter layer are easily obtained, and the cost is low.

Description

[0001] The present invention is a divisional application with the title of "Using White Loam as a Filter Layer to Produce Mushroom and Agaricus Blazei Grain Strains", the filing date of which is January 09, 2012, and the filing number is 201210013247.8. technical field [0002] The invention relates to a technology for producing edible fungus strains, in particular to a method for preparing mushroom and Agaricus blazei strains by using white loam as a filter layer. Background technique [0003] As we all know, the key prerequisite for the success of artificial cultivation of edible fungi such as mushrooms and Agaricus blazei is the strain. Only excellent strains can achieve high quality and high yield. , that is to purify and rejuvenate from the fruiting body to obtain the mother species (also known as the first-class species); the second stage is to produce the original species, that is, to multiply the mother species to the original species, that is, to use the mycelium of ...

AI Technical Summary

Problems solved by technology

Because the fermentation material itself is sterile, after the bacteria are inserted, the mycelia will quickly cover the upper part of the entire bottle while eating the material, and it is easy to form a co-growth advantage to inhibit miscellaneous bacteria. This method can greatly reduce the pollution rate , the disadvantage is that the operation is complicated, time-consuming and labor-intensive

[0006] Although the use of the above-mentioned measures to reduce the contamination rate of strains generally has a good effect of improving the yield of strains, due to the traditional production of mushrooms and Agaricus blazei and other original species (secondary species) and cultivated species (tertiary species) In the process of fermented rice straw or cotton husks or cow dung, which are easy to retain moisture, the fermented material is used as the inoculation block of the mother seed or the original seed to form a mycelium culture substrate after germination, and these mycelium culture substrates are used as guides. Primer (Note: These inoculation block materials that form mycelium culture substrates are often called guide materials), which will redirect mycelium from the original inoculation block to the new cultivation substrate to form a co-growth advantage (Note : The material as the guide material is generally some fermented material which is rich in nutrition and has a good moisturizing ability, which is specially fermented to form a co-growth advantage). The production process of the species, especially in the inoculation process, is difficult to achieve a true aseptic operation state, which has a great negative impact on the common practice of using the co-growth advantage of the strain hyphae to inhibit the growth of miscellaneous bacteria, not only reducing the bacteria The pollution rate is limited and the operation is cumbersome

[0007] In addition, with the development of science, the traditional dung grass strains (also known as dung grass cultivars) used for edible mushrooms such as mushrooms and Agaricus blazei have been on the verge of being eliminated, and the production of mushrooms, Agaricus blazei, etc. As the original species and cultivars for production, wheat grain strains emerged as the times require. Edible fungus such as mushrooms and Agaricus blazei have short production cycle, simple process, consistent bacterial age, strong stress resistance, convenient sowing, and not easy to age. It is more resistant to storage and transportation, so it is more important to use the above-mentioned measures to reduce the pollution rate when making wheat grain strains, but it is also difficult to achieve true aseptic operation in the production process, and often due to guidance The material is rich in nutrients, and the mycelium of the miscellaneous bacteria also eats the material and grows very quickly, thus greatly weakening the original co-growth advantage. The rate is low, which seriously restricts and affects the production efficiency