Unlock instant, AI-driven research and patent intelligence for your innovation.

A detection method for water-soluble sugar content in mainstream smoke

A technology for water-soluble sugar and detection method, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to accurately measure water-soluble sugar content analysis, lack of guiding significance, etc., and achieve low detection limit and good repeatability. Effect

Active Publication Date: 2015-08-05
CHINA TOBACCO GUANGDONG IND
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these three sugars are relatively high in cigarettes, the flavor of cigarettes is the result of the comprehensive influence of various water-soluble sugars. With the in-depth research on the quality of cigarette products, the above detection methods cannot meet the requirements of accurate measurement of cigarettes. The need for analysis of water-soluble sugar content in air
In short, there is currently a lack of guiding significance for practical application, and there is also a lack of a test method for simultaneously detecting multiple water-soluble sugars in mainstream smoke

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A detection method for water-soluble sugar content in mainstream smoke
  • A detection method for water-soluble sugar content in mainstream smoke
  • A detection method for water-soluble sugar content in mainstream smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 The influence of extraction method on result :

[0050] Prepare a sample to be tested according to the treatment method recorded in S1., use ultrasonic extraction and mechanical vibration extraction, and then perform detection. It can be seen from Table 3 that for the test sample, the extraction amount of the ultrasonic extraction method is more than 16% more than that of the vibration method. The possible reason is that the sugar trapped inside the Cambridge filter is more easily extracted by the solution under the action of ultrasonic waves, while mechanical oscillation cannot fully extract the water-soluble sugar inside the Cambridge filter.

[0051] Table 3: Comparison of ultrasonic extraction and vibration extraction (μg / cigarette)

[0052] sugar shaking extraction ultrasonic extraction Arabic candy 2.36 2.93 Galactose 0.34 0.51 glucose 26.12 34.30 Xylose 1.93 2.24 Mannose 4.53 5.37 fructose 53.5...

Embodiment 2

[0053] Example 2 Standard curve linearity, detection limit:

[0054] Quantification by external standard method. With the concentration (μg / mL) as the abscissa x and the peak area as the ordinate y, perform linear regression to obtain the linear regression equation and correlation coefficient. The results showed that the linearity of the method was good, and the correlation coefficients were all greater than 0.999 (Table 4). The signal-to-noise ratio (S / N) was used as the detection limit of the above detection method. The detection limit of the method is lower than 10 ng / mL, and the sensitivity is high.

[0055] Table 4 The linear equation, correlation coefficient and detection limit of the test method

[0056] sugar linear equation correlation coefficient LOD (μg / L) LOQ (μg / L) Arabic candy y = 0.0051x-0.0260 1.0000 2.47 8.23 Galactose y = 0.0045x-0.0045 0.9998 4.10 13.68 glucose y = 0.0046x +0.3164 0.9993 3.31 11.04 Xy...

Embodiment 3

[0057] The precision of embodiment 3 method:

[0058] According to the sample processing method described in S1., 5 copies of the same sample were processed in parallel and measured on the same day to investigate the intraday precision of the method (Table 5). The same sample was processed and measured 4 times in parallel at an interval of 1 day or more within 5 days to investigate the inter-day precision of the method (Table 6). The intra-day and inter-day precision results of the method were expressed as the relative standard deviation of the sugar content value, which were between 2.46% ~ 4.91% and 2.30% ~ 6.82%, respectively, indicating that the method had good repeatability.

[0059] Table 5 Intraday precision of the method

[0060]

[0061] Table 6 Interday precision of the method

[0062]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a smoke component analysis and detection field, concretely a method for detecting water-soluble sugar content in main stream smoke. The method uses a high-efficiency anion exchange chromatography-pulse ampere detection method, uses a NaOH solution as a mobile phase, and uses four-potential waveform to detect six kinds of sugars in the main stream smoke. Water soluble sugars in the main stream smoke captured on a Cambridge filter are ultrasonically extracted for 45min in a 20mg / L NaN3 solution, without other pretreatment before analysis. Effective separation for 6 kinds of water-soluble sugars is implemented by a CarboPacPA20 anion exchange chromatographic column. Detection of the six kinds of water soluble sugars is limited in a range of 2.47-9.82ug / L, linearly dependent coefficients are all larger than 0.999, within-day precision is less than 5.0%, day to day precision is less than 6.9%, and recovery rates of high, medium and low levels are between 97.1% and 102.4%. The method has advantages of low detection limit and good repeatability, and can be suitable for detection of a plurality of cigarette products.

Description

technical field [0001] The invention relates to the field of smoke component analysis and detection, in particular to a detection method for water-soluble sugar content in mainstream smoke. Background technique [0002] Water-soluble sugar is an important component contained in tobacco itself. At the same time, water-soluble sugar is also added to the cigarette production process as an important component of many flavors and fragrances, and plays an important role in regulating the smoking flavor of tobacco. Smokers experience the flavor characteristics of cigarette products primarily through mainstream smoke. Various chemical components in mainstream smoke combine to form the overall style characteristics of cigarettes, in which water-soluble sugar is an important factor related to the sweet taste of cigarette smoking and the amount of total particulate matter and tar generated in mainstream smoke. Element. During the burning process of cigarettes, water-soluble sugar un...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 黄翼飞胡静
Owner CHINA TOBACCO GUANGDONG IND