Convenient-to-integrate device for achieving PCR (polymerase chain reaction) and operating method

Abstract

The invention discloses a convenient-to-integrate device for achieving a PCR (polymerase chain reaction) and an operating method. The device comprises a temperature control module, a semiconductor cooler module, a heat conduction module, a sample injection module, a power module and a reaction pipeline module, wherein the temperature control module is used for outputting two channels of control signals according to the set temperature parameters to achieve connection or disconnection of forward and reverse currents of a semiconductor cooler; one side of the semiconductor cooler clings to a heat radiating block and the other side of the semiconductor cooler clings to the heat conduction module; and the reaction pipeline module is embedded in the heat conduction module. During experiments, the sample injection module sucks reaction liquid into a reaction pipeline to reach the heat conduction module, and valves at the two ends are closed to complete sample injection and sealing; the temperature control module is started to heat the heat conduction module when the forward current of the semiconductor cooler is connected and to refrigerate the heat conduction module when the reverse current of the semiconductor cooler is connected; and temperature control of the reaction pipeline is achieved through variation of the temperature of the heat conduction module and the PCR is finally achieved in the reaction pipeline.

Description

technical field [0001] This patent relates to a PCR experimental device, in particular to a device and operation method for realizing PCR that are easy to integrate, suitable for a portable and automatic biochemical detection system, and belong to the fields of biology, analytical chemistry and medical detection. Background technique [0002] Polymerase Chain Reaction (PCR) is a rapid nucleic acid amplification technology that realizes the DNA replication process in vitro. It is a cell-free amplification technology that is widely used in modern medicine and biological research. PCR is an enzyme-catalyzed process under thermocycling conditions, through cycles of high-temperature denaturation, low-temperature annealing, and suitable temperature extension, so that the target DNA fragment can be rapidly amplified. [0003] The key external condition to realize PCR technology is rapid and accurate temperature cycle control. At present, the devices used to realize PCR are mainly ...

AI Technical Summary

Problems solved by technology

The number of cycles of this chip is not limited, and the chip size is small. Different micro-reaction chamber arrays can be designed according to different needs for high-throughput PCR amplification, which is convenient for miniaturization and portability. The disadvantage is that bubbles are easily generated during the reaction process. , the recovery loss of the reaction solution is large, and it is not easy to integrate with the sampling system

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