Strain of antagonistic poplar colletotrichumgloeosporioides and application thereof

A technology of anthrax bacteria and anthracnose, applied in the field of microorganisms, can solve the problems of difficult chemical pesticide control and environmental pollution, and achieve the effect of easy cultivation and maintenance, and wide adaptability

Inactive Publication Date: 2013-10-02
BEIJING FORESTRY UNIVERSITY +1
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] One of purpose of the present invention is to provide a bacterial strain of the antagonistic bacteria Bacillus atrophaeus XW2 that can efficiently biologically control poplar anthracnose bacterium for the technical problem that existing poplar anthracnose control exists, the bacterial strain of antagonistic bacteria Bacillus atrophaeus XW2 of the present invention is The biocontrol bacterial strain with high-efficiency antagonistic effect on poplar anthracnose obtained by separation and screening from the leaves of poplar, the application of the bacterial strain of the antagonistic bacteria Bacillus atrophaeus XW2 of the present invention solves the current difficulties in the prevention and control of poplar anthracnose with chemical pesticides and serious environmental pollution However, there is a gap in the existing technology of biological control

Method used

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  • Strain of antagonistic poplar colletotrichumgloeosporioides and application thereof
  • Strain of antagonistic poplar colletotrichumgloeosporioides and application thereof
  • Strain of antagonistic poplar colletotrichumgloeosporioides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening and identification of antagonistic bacteria Bacillus atrophaeus XW2

[0045] 1. Screening of antagonistic bacteria Bacillus atrophaeus XW2 against poplar anthracnose

[0046] The bacterial strain of the present invention is collected from healthy poplars in Datangshan Village, Changping District, Beijing.

[0047] 1A) Take 1.5g of freshly collected healthy poplar leaves, put them in a sterilized mortar filled with 10mL of sterile water, add a little sterilized quartz sand to grind evenly, and let it stand for 15min. Take 1mL of suspension and dilute sequentially with 10-fold serial dilution method to obtain 10 -3 、10 -4 、10 -5 Concentration gradient dilution, take 0.1mL from each concentration gradient suspension, spread it on the LB medium plate with a sterile applicator stick, use sterile water as the control, put it in an incubator and invert it at a constant temperature of 28°C after smearing evenly Culture; LB medium (1000mL): tryptone 10g, ...

Embodiment 2B

[0062] Determination of the utilization of different carbon sources and nitrogen sources by the Bacillus atrophaeus XW2 strain of embodiment 2

[0063] 1. Determination of the utilization of different carbon sources by Bacillus atrophaeus XW2 strain

[0064] 1A) Referring to the method in the "Common Bacteria System Identification Manual", use the carbon source basal medium (each 1000mL contains (NH 4 ) 2 SO 4 2.0g, NaH 2 PO 4 ·H 2 O0.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.2g, CaCl 2 2H 2 (00.1g, pH7.0-7.2), adding different carbon sources respectively, the final concentration of carbon sources is generally 0.1% to 0.2% except for sugar alcohols which are 0.5% to 1%. carbon source basal medium;

[0065] 1B) Pick an activated Bacillus atrophaeus XW2 bacteria in 1 mL of sterile water and shake and mix to prepare a bacterial suspension;

[0066] 1C) Take 10uL of the bacterial suspension and inoculate them into 5mL test tubes containing carbon source basal medium contain...

Embodiment 3B

[0079] Determination of the culture characteristics of embodiment 3 Bacillus atrophaeus XW2 bacterial strain

[0080] 1. Determination of the optimal medium for the growth of Bacillus atrophaeus XW2 strain

[0081] 1A) Prepare medium

[0082] LB medium composition: tryptone 10g, yeast extract 5g, NaCl 10g, distilled water to adjust the total volume to 1000ml, pH7.0-7.2;

[0083] NB medium composition: peptone 10g, beef extract 3g, NaCl 5g, distilled water to adjust the total volume to 1000ml, pH7.0-7.2;

[0084] PDB medium composition: potato 200g, glucose 20g, distilled water to adjust the total volume to 1000ml, pH7.0-7.2;

[0085] Gaoshi No. 1 medium composition: soluble starch 20g, KNO 3 1g, NaCl0.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O0.01g, distilled water to adjust the total volume is 1000ml, pH7.0-7.2;

[0086] King B2 medium composition: peptone 20g, MgSO 4 ·7H 2 O1.5g, glycerol 10mL, K 2 HPO 4 1.5g, distilled water to adjust the total vol...

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Abstract

The invention discloses a strain of antagonistic bacteria for preventing and controlling poplar colletotrichumgloeosporioides and application thereof. The antagonistic bacteria are bacillus atrophaeus XW2, and the microbial collection number is CGMCC NO. 7689. The bacillus atrophaeus XW2 is obtained through plate face-to-face culturing and screening of poplar endophytes via a flat plate, has an obvious antagonistic effect on the poplar colletotrichumgloeosporioides, can be used for effectively inhibiting the growth of poplar colletotrichumgloeosporioides hypha and germination of spores and preventing and controlling plant diseases caused by the poplar colletotrichumgloeosporioides, is a biological prevention and control potential strain with high protection effect and good environmental safety and has good development and application prospect.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to an antagonistic bacterium which antagonizes plant pathogenic fungi and application thereof, belongs to the field of microorganisms and can be used for plant protection and plant disease prevention and control. Background technique [0002] There are many varieties of poplar, with strong stress resistance and adaptability. It is an important tree species for afforestation, ecological environment construction and social and economic development in my country. [0003] In recent years, poplar diseases have occurred frequently in China, with an average annual occurrence area of ​​more than 6.6 million mu, causing huge economic and ecological losses. Among them, poplar anthracnose is an important leaf disease, and its pathogen is Colletotrichum gloeosporioides. Poplar anthracnose mainly harms the leaves and branches of poplar, causing early leaf fall and dead shoots, resulting in reduced tree vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 田呈明黄华毅马荣梁英梅游崇娟
Owner BEIJING FORESTRY UNIVERSITY
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