Culture medium for establishing pig iPS cell line and culture method thereof

A culture method and culture medium technology, applied in the field of medium for establishing pig iPS cell lines, can solve problems such as immune rejection, and achieve the effect of neat edges
CN103333920AInactive Publication Date: 2013-10-02NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2013-10-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a culture medium for establishing a pig iPS cell line and a culture method thereof. A typical pig iPS cell is obtained in the ninth day through transfection by four transcription factors of OCT4, SOX2, KLF4 and c-MYC and induction culture of the culture medium, and the pig iPS cell can be obtained efficiently. The obtained pig iPS cell clone is flat clone, has a regular edge and is similar to the ES cellular morphology of the human body. According to the pig iPS cell line, the pig iPS cell through subculture keeps the undifferentiated state, shows positive in the AP dyeing displaying result and has a pluripotency mark, and the differentiated cell in vitro expresses NCSTN (entoderm), NESTIN (ectoderm) and DESMIN (mesoblast).
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Description

technical field

[0001] The invention belongs to the technical field of induced pluripotent stem cells, and relates to a culture medium for establishing pig iPS cell lines and a culture method thereof. Background technique

[0002] Pluripotent stem cells (pluripotent stem cells) refer to a type of cells that have the potential to form all types of cells in humans and animals and can continuously renew themselves. Due to the multi-directional differentiation potential and self-renewal characteristics of pluripotent stem cells, it has its unique role and advantages in the research of cell replacement therapy, gene therapy, developmental biology research, pharmacology and toxicology. Currently, the most well-known pluripotent stem cells are embryonic stem cells (ES cells), which are generally cell lines established from the inner cell mass of blastocysts under appropriate in vitro culture conditions. However, the acquisition of ES cells, especially the acquisition of human ES c...

Claims

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