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A nitrogen-fixing lactobacillus and its use

A technology of Lactobacillus sporogenes and nitrogenase, applied in the direction of bacteria, enzymes, biochemical equipment and methods, etc., can solve problems such as hazards, unbearable farmers, non-point source pollution, etc., and achieve the effect of broad application prospects

Active Publication Date: 2015-09-16
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of chemical nitrogen fertilizer consumes a lot of energy, accounting for 70% to 80% of the total production cost
In recent years, energy has become increasingly tense, and the price of coal used in fertilizer production has soared, resulting in an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response
Chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food security in my country, but unreasonable nitrogen application has formed serious non-point source pollution, causing a series of serious hazards, such as the shocking cyanobacteria incident in Taihu Lake

Method used

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  • A nitrogen-fixing lactobacillus and its use
  • A nitrogen-fixing lactobacillus and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Isolation and Identification of Sporolactobacillus sp. GD412CGMCC No.7777

[0041]1. Enrichment and isolation of crop rhizosphere nitrogen-fixing bacteria GD412

[0042] Take 10g soil sample (collected from Liaoning Province, China) and shake it in 90ml sterile water for 20 minutes to make a turbid solution. Take 5ml and put it into 30ml nitrogen-fixing bacteria enrichment culture medium ACCC55, 100rpm, 28℃ for shaking culture, After 72 hours, fresh culture medium was changed to continue cultivation. The nitrogen-fixing spores were isolated after repeating the culture for 3 times. Draw 1ml of the above-mentioned nitrogen-fixing bacteria enrichment culture and put it into 9ml sterile water to make 10 -1 Dilution, continue to dilute to make 10 -2 、10 -3 、10 -4 、10 -5 Bacterial suspensions of dilutions were heated in boiling water at 100°C for 10 minutes, and after cooling, 0.1ml of each dilution was spread on a nitrogen-free medium plate and cultured stat...

Embodiment 2

[0072] Example 2, Determination of Nitrogenase Activity of Sporolactobacillus sp. GD412CGMCC No.7777

[0073] Carry out nitrogenase activity assay to the obtained spore lactobacillus (Sporolactobacillus sp.) GD412CGMCC No.7777 of embodiment 1, specific method is as follows: in 15 * 150mm screw-top glass tubes, add 5mL improved nitrogen-fixing medium to make inclined-plane, Inoculate Lactobacillus sporolactobacillus (Sporolactobacillus sp.) GD412CGMCC No.7777 and culture at 28°C. Azotobacter chroococcum (Azotobacter chroococcum) ACCC11103, which is commonly used in the production of microbial fertilizers, was used as the positive control, and the blank slant without inoculation was used as the negative control. Three replicates were set for each treatment. After culturing for 72 hours, replace the rubber stopper, inject acetylene gas to make the final concentration 10%, seal it with medical adhesive tape, continue culturing for 72 hours, take 100 μL of reaction gas, measure the...

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PUM

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Abstract

The invention discloses nitrogen fixing sporolactobacillus and an application thereof. The nitrogen fixing sporolactobacillus is sporolactobacillus sp. GD412 and the preservation number of the sporolactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.7777. The sporolactobacillus sp. GD412 with the preservation number of CGMCC NO.7777, disclosed by the invention, can be colonized at the rhizosphere of crops and has very high nitrogen fixing potentiality and very broad application prospect in the production of vegetable industrial seedling growing inoculants, nitrogen fixing microbial agents and biological organic fertilizers.

Description

technical field [0001] The invention relates to a nitrogen-fixing lactobacillus and its application. Background technique [0002] Nitrogen fertilizer is one of the most important means of production in agricultural production. The production of chemical nitrogen fertilizer consumes a large amount of energy, accounting for 70% to 80% of the total production cost. In recent years, energy has become increasingly tense, and the price of coal used in fertilizer production has soared, which has led to an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response. Chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food security in my country, but unreasonable nitrogen application has formed serious non-point source pollution, causing a series of serious hazards, such as the shocking cyanobacteria incident in Lake Tai. Nitrate pollution in urban groundwater has threatened the safety o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/00C05F11/08C12R1/01
Inventor 孙建光张燕春杨慧
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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