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Nitrogen-fixing arthrobacterium and application thereof

An Arthrobacter, nitrogenase technology, applied in bacteria, organic fertilizers, oxidoreductase and other directions, can solve problems such as harm, non-point source pollution, chemical nitrogen fertilizer prices, etc., and achieve the effect of broad application prospects.

Active Publication Date: 2013-10-02
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of chemical nitrogen fertilizer consumes a lot of energy, accounting for 70% to 80% of the total production cost
In recent years, energy has become increasingly tense, and the price of coal used in fertilizer production has soared, resulting in an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response
Chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food security in my country, but unreasonable nitrogen application has formed serious non-point source pollution, causing a series of serious hazards, such as the shocking cyanobacteria incident in Taihu Lake

Method used

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  • Nitrogen-fixing arthrobacterium and application thereof
  • Nitrogen-fixing arthrobacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Isolation and identification of Arthrobacter sp. GD601CGMCC No.7779

[0041] 1. Enrichment and isolation of crop rhizosphere nitrogen-fixing bacteria GD601

[0042]Take 10g soil sample (collected from Inner Mongolia Autonomous Region, China) and shake it in 90ml sterile water for 20 minutes to make a turbid solution. Take 5ml and put it into 30ml nitrogen-fixing bacteria enrichment culture medium ACCC55, 100rpm, 28℃ for shaking culture, After 72 hours, fresh culture medium was changed to continue cultivation. The nitrogen-fixing spores were isolated after repeating the culture for 3 times. Draw 1ml of the above-mentioned nitrogen-fixing bacteria enrichment culture and put it into 9ml sterile water to make 10 -1 Dilution, continue to dilute to make 10 -2 、10 -3 、10 -4 、10 -5 Bacterial suspensions of dilutions were heated in boiling water at 100°C for 10 minutes, and after cooling, 0.1ml of each dilution was spread on a nitrogen-free medium plate and cult...

Embodiment 2

[0072] Example 2, Arthrobacter sp. GD601CGMCC No.7779 Nitrogenase Activity Determination

[0073] Arthrobacter sp. GD601CGMCC No.7779 obtained in Example 1 is carried out to measure the nitrogenase activity, and the specific method is as follows: add 5 mL of improved nitrogen-fixing medium to make a slant in a 15 × 150 mm screw-top glass tube, and inoculate Arthrobacter sp. GD601CGMCC No.7779 was cultured at 28°C. Azotobacter chroococcum (Azotobacter chroococcum) ACCC11103, which is commonly used in the production of microbial fertilizers, was used as the positive control, and the blank slant without inoculation was used as the negative control. Three replicates were set for each treatment. After culturing for 72 hours, replace the rubber stopper, inject acetylene gas to make the final concentration 10%, seal it with medical adhesive tape, continue culturing for 72 hours, take 100 μL of reaction gas, measure the amount of ethylene produced by gas chromatography, and calculate ...

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Abstract

The invention discloses a nitrogen-fixing arthrobacterium and application thereof. The nitrogen-fixing arthrobacterium is Arthrobacter sp. GD601, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7779. The Arthrobacter sp. GD601 CGMCC No.7779 provided by the invention can be colonized in rhizosphere of a crop, and has a quite high nitrogen-fixing potential; the nitrogen-fixing arthrobacterium has a wide application prospect in inoculation agent, microbial agent and bio-organic fertilizer production for vegetable industrialized seedling production.

Description

technical field [0001] The invention relates to a strain of nitrogen-fixing Arthrobacter and its application. Background technique [0002] Nitrogen fertilizer is one of the most important means of production in agricultural production. The production of chemical nitrogen fertilizer consumes a large amount of energy, accounting for 70% to 80% of the total production cost. In recent years, energy has become increasingly tense, and the price of coal used in fertilizer production has soared, which has led to an increase in the price of chemical nitrogen fertilizers, which is unbearable for farmers and has a strong response. Chemical nitrogen fertilizers have played a huge role in increasing crop yields and ensuring food security in my country, but unreasonable nitrogen application has formed serious non-point source pollution, causing a series of serious hazards, such as the shocking cyanobacteria incident in Lake Tai. Nitrate pollution in urban groundwater has threatened the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/02C05F11/08C12R1/06
Inventor 孙建光罗琼周义清
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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