A test strip and method for detecting streptomycin
A technique for streptomycin and test strips, which is applied to the field of colloidal gold test strips for detecting streptomycin in samples, can solve the problems of being unsuitable for large-scale on-site detection, complicated processing procedures, and high degree of instrumentation, and can achieve the detection time. Short, easy-to-operate, high-sensitivity effects
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Embodiment 1
[0041] Embodiment 1 detects the composition of the test strip of streptomycin
[0042] 1. Test paper ( figure 1 )
[0043] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;
[0044] The sample absorbent pad 1, the reaction film 2, the water absorbent pad 3 and the protective film 7 are pasted on the bottom plate 6 in sequence, the end of the sample absorbent pad is connected with the reaction film, the end of the reaction film is connected with the water absorbent pad, and the sample absorbent pad The beginning is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;
[0045] The sample absorption pad end of the test paper is pasted with a protective film, and the protective film 7 covers the detection end on the sample absorption pad, and the word MAX is printed on the detection end protective film ( figure 2 );
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Embodiment 2 Embodiment 1
[0051] The preparation method of test strip described in embodiment 2 embodiment 1
[0052] 1. Preparation of test strips
[0053] The preparation method of test strip mainly comprises the following steps:
[0054] 1) Prepare freeze-dried microporous reagents with streptomycin monoclonal antibody-colloidal gold markers;
[0055] 2) preparing a reaction membrane with a detection area coated with a streptomycin hapten-carrier protein conjugate and a quality control area coated with a goat anti-mouse anti-antibody;
[0056] 3) Assemble the reaction film prepared in 2) with the sample absorbent pad, water absorbent pad, protective film, and bottom plate into a test paper;
[0057] 4) Assemble the freeze-dried microporous reagent and test paper prepared in 1) and 3) with streptomycin monoclonal antibody-colloidal gold markers into a test strip.
[0058] The following step-by-step detailed description:
[0059] (1) Preparation of each component
[0060] 1. Synthesis and identific...
Embodiment 3
[0098] The detection of streptomycin in the sample of embodiment 3
[0099] 1. Sample pretreatment
[0100] When testing honey samples, it is necessary to pre-treat the honey samples:
[0101] Accurately weigh 0.5g of honey into a 4ml centrifuge tube, add 1.5ml of 0.2mol / L phosphate buffer, vortex for 1min, and mix well for testing. (Note: 1. The honey without crystallization needs to be stirred evenly before weighing; 2. If there is crystallized honey, put it in a water bath not higher than 60°C to warm it up, wait until the honey is completely melted, stir evenly, and cool it to room temperature before weighing. weighing.)
[0102] 2. Test samples with test strips
[0103] Testing Milk Samples
[0104] Take out the required number of microwell reagents and test papers from the original package, and mark them; draw the milk sample to be tested, pipette 200 μl into the microwell with a micropipette, slowly suck and fully mix with the reagent in the microwell Evenly, incub...
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