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Method for loading tumor antigen peptide to dendritic cell (DC) in targeting manner

A tumor antigen peptide, dendritic cell technology, applied in the medical field, can solve problems such as immune stagnation, and achieve the effect of improving ability

Inactive Publication Date: 2013-11-27
扬州维克斯生物科技有限公司
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

The danger of this operation is that tumor antigens are self-antigens. Simple and low-efficiency loading of self-antigens will not only fail to induce immune responses, but will lead to immune stagflation, which is counterproductive.

Method used

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  • Method for loading tumor antigen peptide to dendritic cell (DC) in targeting manner
  • Method for loading tumor antigen peptide to dendritic cell (DC) in targeting manner
  • Method for loading tumor antigen peptide to dendritic cell (DC) in targeting manner

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Embodiment Construction

[0021] For the experimental methods not indicated in the following examples, the specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the corresponding raw material manufacturers.

[0022] 1. Preparation and biotin labeling of fusion protein V:

[0023] 1. Construction of recombinant gene expression vector:

[0024] Specific measures for recombining the anti-DEC-205 antibody molecule and the tumor antigen peptide MUC1 in the same fusion protein:

[0025] According to the functional diagram and the corresponding nucleic acid sequence, using the method of commercial artificially synthesized nucleic acid, synthesize 744 bases of the antibody molecule (antiDEC-ScFV) with anti-DEC-205, 72 bases of the MUC1 polypeptide (MUC1) and expression Purify 69 bases of the required functional region (TEV, Myc, His6). The synthetic whole gene fragment (855 bases) was cloned into the commercial plasmid vector pET41a(+) b...

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Abstract

The invention provides a method for loading a tumor antigen peptide to a dendritic cell (DC) in a targeting manner, and belongs to the field of medicine technology. The method is characterized in that a fusion protein with antibody activity is tightly coupled with a DC surface receptor molecule DEC-205, and a tumor antigen peptide MUC1 is loaded to the DC in a targeting manner, so that the capacity of the DC in terms of the presentation of the tumor antigen peptide MUC1 can be greatly improved.

Description

technical field [0001] The invention belongs to the field of medical technology, in particular to a method for treating tumor antigen targeted loading in tumor biotechnology. Background technique [0002] Tumor biological therapy is the fourth type of cancer treatment developed after surgery, radiotherapy and chemotherapy. It uses the immune response stimulated by the body to fight, inhibit and kill cancer cells. Different from traditional treatment methods, tumor biotherapy is based on cutting-edge science such as modern molecular biology, cell biology and molecular immunology, and uses the body's immune response to tumors to change the interaction between tumors and the body through biological means. , mobilize the body's defense mechanism against tumors, achieve the purpose of controlling tumor growth, prolonging the survival period of patients, and improving the quality of life of patients. It has become an important treatment method in the comprehensive treatment of tum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/70C12N5/0784C07K19/00
Inventor 胡伟明夏冰
Owner 扬州维克斯生物科技有限公司
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