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Application of human miR-26b in preparation of drugs for inhibiting fat cell proliferation

A mir-26b, fat-inhibiting technology, applied in the field of genetic engineering, can solve the unknown relationship between miRNA and adipocyte proliferation

Inactive Publication Date: 2015-06-10
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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Problems solved by technology

But how this miRNA is related to fat cell proliferation is unknown

Method used

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  • Application of human miR-26b in preparation of drugs for inhibiting fat cell proliferation
  • Application of human miR-26b in preparation of drugs for inhibiting fat cell proliferation
  • Application of human miR-26b in preparation of drugs for inhibiting fat cell proliferation

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Embodiment 1

[0016] 1 Materials and methods

[0017] 1.1 Test materials: Human Preadipocytes-visceral (HPA-v) was purchased from Sciencell, USA. The pGLV-H1-GFP / Puro plasmid required for this experiment was purchased from Shanghai Gemma Company, and the packaging plasmids Pcgvp, Rev, and Vsvg were purchased from Allele Biotech & Pharmaceuticals in the United States; the pGLV-H1-miR-26b-GFP / Puro lentiviral vector Self-constructed by our experimental group; DNA and RNA extraction kits were purchased from Qiagen, Germany; miRNA reverse transcription kits, miRNA detection kits, and TanMan gene expression mix II were purchased from ABI, USA; TaKaRa LA DNA Polymerase Hot-Start Version PCR Kit was purchased from Takara Company of Japan, Adipocyte Proprietary Medium 7211 was purchased from Sciencell Company of the United States, restriction endonuclease and T4 ligase were purchased from NEB Company of the United States, and primers were synthesized by Invitrogen Company of the United States.

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Abstract

The invention belongs to the field of gene engineering and discloses the application of human miR-26b in the preparation of drugs for inhibiting fat cell proliferation. The invention provides a technical scheme of applying human miR-26b in the preparation of drugs for inhibiting fat cell proliferation. The study results provided in the invention show that the over-expression of human miR-26b in human fat cells can significantly inhibit the proliferation velocity and cell quantity of fat cells, and human miR-26b can be used to prepare drugs for inhibiting fat cell proliferation and drugs for weight loss.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to the application of human miR-26b in the preparation of drugs for inhibiting fat cell proliferation. Background technique [0002] Obesity is a common energy metabolism disease and the fastest growing chronic disease worldwide. Obesity is closely related to various diseases such as coronary heart disease, hypertension, hyperlipidemia, diabetes, and cerebrovascular accident, which seriously endanger the physical and mental health of patients. Strengthening research on the etiology, pathogenesis and prevention and treatment of obesity has become one of the biggest public health challenges in the 21st century. Current obesity treatment strategies include appetite suppressants, digestive lipase inhibitors, and hormone modulators that stimulate central adrenergic receptors or prevent serotonin reuptake. Of these drugs, lipase inhibitors, orlistat and sibutramine, which only block sero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P35/00A61P3/04
Inventor 郭锡熔季晨博赵亚萍宋桂仙徐广峰史春梅顾平清沈亚卉李慧
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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