Prediction method for retention time of high performance liquid chromatographic peak
A high-performance liquid chromatography, retention time technology, applied in the field of high-performance liquid chromatography peak retention time prediction
Inactive Publication Date: 2013-12-11
NAT INST FOR FOOD & DRUG CONTROL
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Problems solved by technology
However, due to the difference between the chromatographic instrument and the chromatographic column, the deviation between the predicted value of the retention time and the measured value is often large. In practical applications, it is necessary to limit the type of chromatographic column, or choose the same type of chromatographic column with similar separation performance. inconvenient
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[0053] This embodiment provides a high performance liquid chromatography peak retention time prediction method, which performs prediction of the chromatographic peak retention time of rhubarb, including the following steps:
[0054] 1. Column fitting:
[0055] According to the optimized chromatographic conditions (according to the "Chinese Pharmacopoeia" 2010 edition a rhubarb content determination items (page 22), the specific parameters are methanol-0.1% phosphoric acid aqueous solution (85:15), detection wavelength 254nm, column Temperature 30°C, flow rate 1.0mL min -1 ), mixed reference substance or test solution on 11 C18 columns of different brands and models, and obtained five components (aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether) in different For the measured retention time on the chromatographic column, inject at least three times and keep three decimal places, and remove the chromatographic column with RSD greater than 2%; there are 12 chromatogr...
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The invention relates to a prediction method for retention time of a high performance liquid chromatographic peak. The method comprises the following steps: measuring standard retention time of a variety of components of a variety of samples; choosing two components from target components of each of the samples as dual standard control components of the sample; measuring actual retention time of the dual standard control components in a test solution of a to-be-measured sample; measuring actual retention time of other target components; carrying out two-point verification and multi-point verification; etc. With the prediction method, retention time of chromatographic peaks of a variety of components in the to-be-measured sample can be accurately predicted, then nature determination is carried out on the chromatographic peaks of the to-be-measured sample, and discrimination of the to-be-measured sample is carried out. The method provided by the invention has high prediction precision, is applicable to more chromatographic columns and is obviously superior to relative retention time methods in the prior art.
Description
technical field [0001] The invention relates to a high performance liquid chromatography peak retention time prediction method, which belongs to the technical field of high performance liquid chromatography detection. Background technique [0002] One of the core tasks in the content determination of multi-index components of traditional Chinese medicine, fingerprint analysis, and related substance inspection of chemical drugs (or antibiotics) is to characterize a large number of target compounds. If pure chemical reference substances are used, the standard The preparation and supply of substances bring great difficulties and will also significantly increase the cost of testing. Therefore, it is of great significance and application value to use a small amount of chemical reference substances to characterize other chromatographic peaks by a certain method. [0003] At present, the relative retention time method using a chemical reference substance is the most commonly used ...
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IPC IPC(8): G01N30/86G01N30/91
Inventor 孙磊金红宇马双成
Owner NAT INST FOR FOOD & DRUG CONTROL