Biological agent for inducing dentin mineralization

A technology of biological preparations and dentin, applied in the field of tooth mineralization drugs in oral biology, can solve problems affecting the mineralization process of dentin, and achieve the effect of inhibiting the development process

Inactive Publication Date: 2013-12-18
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So, the study that pyrrolidine dithiocarbamate affects the dentin mineralization process by regulating the expression of these cytokines has not been reported yet.

Method used

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  • Biological agent for inducing dentin mineralization
  • Biological agent for inducing dentin mineralization
  • Biological agent for inducing dentin mineralization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] First, isolate and culture human dental pulp cells and dental pulp stem cells. The specific steps are as follows: clinically collect healthy and complete permanent teeth freshly extracted from volunteers aged 18 to 25 (informed consent) due to orthodontic reduction or impaction. Take out the pulp tissue, wipe the surface of the tooth with 750mL / L ethanol, then soak it twice with PBS buffer (phosphate buffer) containing penicillin and streptomycin; Split, take out the pulp in the ultra-clean bench, put it in PBS buffer solution, and cut the pulp into 1mm with ophthalmic scissors 3 ~2mm 3 After centrifuging at 1000r / min for 5min, discard the supernatant; then add 4mg / LI type collagenase to digest in a water bath at 37°C for 45min; use a centrifuge (Kubota2l00, Japan) to centrifuge at 1000r / min for 5min, discard the supernatant ; Wash 3 times with α-MEM medium containing 50mL / L fetal bovine serum. Then, after centrifugation at 1000r / min for 5min, the cells were collecte...

Embodiment 2

[0042] Pyrrolidine dithiocarbamate-induced dental pulp stem cells were co-incubated with calcined bone bio-scaffolds and transplanted into nude mice to further observe the effect of pyrrolidine dithiocarbamate on promoting dentin mineralization.

[0043] First, prepare calcined bovine bone: deproteinize fresh bovine cancellous bone with NaOH, calcinate at 850°C for 4 hours, pulverize and sieve, make 200-280μm particles, sterilize with ethylene oxide, and set aside.

[0044] Collagen preparation: prepare 0.1% v / v glacial acetic acid and 10% NaOH solution, filter and sterilize, and set aside; use 0.1% v / v glacial acetic acid to dilute the purchased 8 mg / ml collagen to 6 mg / ml, and dilute the obtained collagen , 10×DMEM, and fetal bovine serum are mixed in a volume ratio of 10:1:1, and the pH value is adjusted to be neutral with 10% NaOH solution; the collagen solution is divided into sterile 1.5ml EP tubes, 500ul per tube , take out 3 of the EP tubes, dissolve PDTC in 6mg / ml ste...

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Abstract

The invention discloses a biological agent for inducing dentin mineralization. The biological agent comprises pyrrolidine dithiocarbamate. A method for preparing the biological agent in unit dose comprises the following steps: mixing 20umol / L of pyrrolidine dithiocarbamate, 1*10<-8>mol / L of dexamethasone, 10mmol / L of sodium-beta-glycerophosphate and 50ug / mL of vitamin C, and then adding 100mL / L of alpha-MEM culture solution of fetal bovine serum, thus preparing the biological agent. The biological agent can promote the biological mineralization process of hard tissues of teeth and effectively form a reparative dentinal bridge, thus inhibiting the occurrence and development processes of caries.

Description

technical field [0001] The invention relates to the field of oral biology tooth mineralization drugs, in particular to a biological preparation for inducing dentin mineralization. Background technique [0002] Caries is listed by the World Health Organization as one of the three major non-communicable diseases (cardiovascular disease, cancer and dental caries) that endanger human health. The basic changes in tooth tissue during caries are the demineralization of inorganic matter and the decomposition of organic matter. Under the control of various biological factors, inorganic ions such as calcium and phosphorus in and out of the organism can produce insoluble salts through chemical reactions and combine with organic matrices to form mineralized tissues. This biomineralization process of dental hard tissue can reverse or slow down the development of dental caries to some extent. This also plays an important role in the repair process of pulp damage. At the same time, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/02
Inventor 何文喜王志华余擎吕海鹏周泽渊付蕾马逢乐王娟
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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