Preparation of zirconium dioxide and ferriferrous oxide nanoparticles and method used for enrichment of phosphopeptides by using zirconium dioxide and ferriferrous oxide nanoparticles

Abstract

Calcium deficiency and low absorptivity of calcium are known as global problems, and it is confirmed that phosphorylated peptides are capable of promoting absorption of calcium by human body effectively, so that research on high-efficient and rapid enrichment method of phosphopeptides is of significant importance. Separation and purification of target substances by taking magnetic nanoparticles as adsorption carriers are emerging technologies. According to the emerging technologies, the magnetic nanoparticles are subjected to surface modification so as to realized separation and purification of the target substances, and rapid separation of the target substances from complex systems can be realized in the presence of an applied magnetic field. Compared with traditional methods, magnetic separation technology is fast, is simple in operation, and high in efficiency and recovery rate. The invention firstly provides a preparation method of metallic oxide magnetic nanoparticles, and the obtained zirconium dioxide and ferriferrous oxide magnetic nanoparticles are used for enrichment of phosphopeptides. The zirconium dioxide and ferriferrous oxide magnetic nanoparticles possess superparamagnetism and excellent water dispersibility, and show high specificity on adsorption of phosphopeptides. Enrichment effects on phosphopeptides of the method based on the metallic oxide magnetic nanoparticles are obviously better than that of the conventional methods.

Description

technical field [0001] The invention relates to the technical field of nanomaterials and protein separation and purification, in particular to the preparation of zirconium dioxide ferric oxide nanoparticles and a method for enriching phosphopeptides. Background technique [0002] Calcium is the most abundant trace element in the human body. It is called the "environmental guard" of the human body. It has important physiological functions, including: mildly inhibiting hyperthyroidism, preventing ectopic calcification and ossification, reducing the recurrence rate of colon cancer and Reduce the incidence of hypertension. Long-term calcium deficiency can lead to underdeveloped bones and teeth, osteoporosis, anemia, and even high blood pressure and coronary heart disease. The average daily calcium intake of Chinese residents is only about 400 mg, which only accounts for about 50% of the daily recommended amount, so calcium supplementation is a top priority. [0003] In 1950, M...

AI Technical Summary

Problems solved by technology

Enrichment of phosphopeptides by using the prepared zirconia ferroferric oxide nanoparticles is easy to operate, low in cost, short in production cycle, and easy for large-scale industrial production, which solves the problems of traditional phosphopeptide enrichment difficulties and numerous steps.

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