Eupenicillium cinnamopurpureum strain and application thereof

A technology of Penicillium baizheng and cinnamon, which is applied in the field of phosphorus-dissolving microbial fertilizers, can solve complex problems, achieve the effects of enhancing absorption rate, strong phosphorus-dissolving ability, and improving soil structure

Inactive Publication Date: 2013-12-25
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of fungal phosphorus dissolution is very complicated. Some fungal phosphorus dissolution is mainly caused by protons, some mainly by organic acids, while some strains have both mechanisms, and some strains produce other chelating substances during the growth process. lead to the dissolution of phosphate rock powder

Method used

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  • Eupenicillium cinnamopurpureum strain and application thereof
  • Eupenicillium cinnamopurpureum strain and application thereof
  • Eupenicillium cinnamopurpureum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Isolation and purification of E.cinnamopurpureum WHP-1

[0029] Penicillium cinnamopurpureum (E.cinnamopurpureum) WHP-1 of the present invention is obtained from the separation of the soil by the dilution plate method and the plate streak method, and the separation method is:

[0030] (1) Isolation of the Penicillium cinnamon cinnamomi strain: Soil samples were collected from plots planted with different types of crops in different areas of coastal wetlands in Shandong, and the 5-point sampling method was used to collect the surrounding and central depths of the plots within the range of 10-20cm. Appropriate amount of soil, mix in equal amount. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL of sterile water, place in a shaker at 30°C at 150rpm for 10min, take 100μL of 10 -2 、10 -3 、10 -4 The dilution was spread on the PDA medium plate, and each gradient was spread in three parallels. After culturing at 30°C for 2 days...

Embodiment 2

[0042] 1. Fermentation process of Penicillium cinnamomi Cinnamonensis strain WHP-1

[0043] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0044] A large amount of solid fermentation medium formula (mass percentage):

[0045] Solid material: rice bran 85%, bran 15%;

[0046] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, and the rest is water.

[0047] The solid-to-liquid ratio is 1:1.8 (mass ratio).

[0048] Mass solid fermentation process of E.cinnamopurpureum (E.cinnamopurpureum) WHP-1:

[0049] ①Cultivation of strains in seed solution: Pick a small amount of spores of E.cinnamopurpureum WHP-1 from the inclined surface of the test tube, transfer them to PDB liquid medium, and culture them on a shaker at 28°C for 3-5 days. This is the seed solution .

[0050] ②Cultivation of solid production strains: inoculate 10% of the seed solution into solid medium (500mL Erlenmeyer flask)...

Embodiment 3

[0052] Example 3 Trial of E.cinnamopurpureum (E.cinnamopurpureum) WHP-1 rice plots

[0053] Test material: E.cinnamopurpureum (E.cinnamopurpureum) WHP-1 fermentation liquid (seed liquid obtained in step ①), which has the effect of dissolving phosphorus, and the effective number of viable bacteria is greater than 2.5×10 8 cfu / ml.

[0054] Test time: from June 2012 to November 2012

[0055] Test location: Dujiazhuang Village, Yanbin Town, Junan County

[0056] Test crop: Lindao 16

[0057] Test soil: clay

[0058] Experimental design: Dressing rice seeds with different dilutions (10 times, 20 times, 30 times) of Penicillium cinnamon purpureum (E.cinnamopurpureum) WHP-1 as a demonstration, and dressing rice seeds with substrate medium as a control (CK ), three parallels for each treatment, the area of ​​the plot is 20m 2 , randomly distributed.

[0059] Fertilization amount and fertilization method: Before sowing, use different dilutions (10 times, 20 times, 30 times) of Pe...

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Abstract

The invention discloses eupenicillium cinnamopurpureum strain and an application thereof, and belongs to the technical field of P-solubilizing microbial fertilizers. The bacterial strain is eupenicillium cinnamopurpureum WHP-1, and the preservation number is CGMCC No. 8128. The invention also discloses a microbial inoculum containing the bacterial strain and a preparation method thereof. Experiments show that the eupenicillium cinnamopurpureum strain WHP-1 has the characteristics of fast growth speed, large sporulation quantity, strong stress resistance, strong P-solubilizing performance and the like, and can be fast colonized in quantity at the plant roots. The fermentation broth or the microbial inoculum of the bacterial strain contains a large amount of P-solubilizing bacterium, and has certain active effect on soil improvement; and due to the P-solubilizing function, the fermentation broth or the microbial inoculums has active influence on improvement of soil structure, enhancement of fertilizer absorptivity of crops, and the like.

Description

technical field [0001] The invention belongs to the field of phosphorus-dissolving microbial fertilizers. Specifically, the present invention relates to a high-efficiency Penicillium cinnamona cinnamona fungus and its application, and a fungal agent prepared by using the Penicillium cinnamona . Background technique [0002] Phosphorus is the basic constituent element of genetic material DNA and energy material ATP in plant cells, and plays an important role in the whole life process of plants. Phosphorus is one of the essential elements for plant growth and development. The high-energy phosphate bond is the carrier of energy. Phosphorus must be involved in the photosynthesis of plants and the biochemical processes in the body. Phosphorus is not only an important material guarantee for agricultural production, but also a non-renewable mineral resource. The mass fraction of total phosphorus in cultivated soil in my country is generally 0.2-1.lg / kg -1 (soil) changes, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/00C12R1/645C09K101/00
Inventor 刘幸红牛赡光王清海段春华郑香军周峰
Owner SHANDONG FOREST SCI RES INST
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