Streptomyces albidoflavus strain and application thereof to control cucumber downy mildew

A technology of Streptomyces albicans and cucumber downy mildew, which is applied in the field of biological control preparations, can solve the problems of environmental pollution, long cycle of disease-resistant varieties, single resistance, etc., and achieve the goal of increasing crop yield, good application prospects, and fast growth Effect

Inactive Publication Date: 2013-12-25
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure

Method used

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  • Streptomyces albidoflavus strain and application thereof to control cucumber downy mildew
  • Streptomyces albidoflavus strain and application thereof to control cucumber downy mildew
  • Streptomyces albidoflavus strain and application thereof to control cucumber downy mildew

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Isolation and purification of S. albidoflavus ACT033

[0030] The S. albidoflavus ACT033 of the present invention is obtained from the soil by the dilution plate method and the plate streak method. The separation method is:

[0031] (1) Separation of streptomyces: soil samples are collected, plots of different crop types are selected in different areas along the beach, and a 5-point sampling method is adopted to collect appropriate amounts of soil around the plot and the central depth within the range of 10-20cm. , Mix the same amount. Indicate the location, time and person of collection. Weigh 1g soil sample in 100mL sterile water, place it in a 30℃ shaker, shake at 150rpm for 10min, and take 100μL 10 -2 , 10 -3 , 10 -4 The dilution was spread on the Gao's No. 1 medium plate, and each gradient was spread in three parallels. After incubating at 30°C for 2 days, the microbial colonies of different forms on the Gao's No. 1 medium were picked and cultured on the Gao's No. 1...

Embodiment 2

[0066] 1. Fermentation process of Streptomyces flavus ACT033

[0067] Millet liquid culture medium: The formula is millet 10g, add 10g of glucose, 2g of sodium chloride, 2g of peptone, 2g of calcium carbonate, add 10g of millet to 1000ml of distilled water and boil for 30min, add distilled water to 1000ml, and adjust the pH to 7.2-7.4.

[0068] A large number of solid fermentation medium formula (quality percentage):

[0069] Solid material: 70% corn flour, 30% bran

[0070] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, and the rest is water.

[0071] The solid-liquid ratio is 1:1.8 (mass ratio)

[0072] S. albidoflavus ACT033 mass solid fermentation process:

[0073] ①Bacterial seed liquid culture: Pick a small amount of bacteria from the slope of the test tube from S. albidoflavus ACT033, move them to the millet liquid medium, and culture in a shaker at 30°C for 3 to 5 days. This is the seed liquid.

[0074] ② Cultivation of s...

example 3

[0076] Example 3 Test report on the ability to control cucumber downy mildew virus

[0077] The invention is a new type fungicide developed by Shandong Academy of Forestry Sciences, which can use the spore liquid of Streptomyces flavus ACT033 to achieve the purpose of cucumber downy mildew, and can be completely degraded in nature and has no adverse side effects on the ecological environment. The virulence of the developed 20 million / g Streptomyces flavus ACT033 fermentation broth (aqueous agent, the seed broth of the above step (1)) was tested against cucumber downy mildew, and the EC was calculated 50 , Now the report is as follows:

[0078] 1Materials and methods

[0079] 1.1 Material

[0080] a. Test strain: Cucumber downy mildew was provided by the Institute of Plant Protection, Shandong Academy of Agricultural Sciences.

[0081] b. Cucumber varieties: Lucucumber No. 10, provided by the Vegetable Research Institute of Shandong Academy of Agricultural Sciences.

[0082] c. Test agen...

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Abstract

The invention discloses streptomyces albidoflavus strain and an application thereof to control cucumber downy mildew, and belongs to the field of biological control on plant diseases. The bacterial strain is streptomyces albidoflavus ACT033, and the preservation number is CGMCC No. 8125. The invention also discloses a biological control preparation which is prepared by employing the new streptomyces albidoflavus strain ACT033 and is capable of effectively controlling cucumber downy mildew and a preparation method of the preparation. Experiments show that the streptomyces albidoflavus strain ACT033 of the invention has the characteristics of fast growth speed, large sporulation quantity, broad-spectrum effect, strong stress resistance and the like, and can be fast colonized in quantity at the plant roots, so good application prospect is achieved. The biological control preparation prepared from the streptomyces albidoflavus strain ACT033 not only can control cucumber downy mildew, but also can effectively increase crop output.

Description

Technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a strain of Streptomyces leucocephalus that can effectively control cucumber downy mildew, and a biological control agent prepared by using the Streptomyces leucocephalus. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop deaths, causing significant economic losses. For the control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. Disease-resistant varieties have a long cycle and single resistance. The long-term use of chemical control agents and the large amount of use have caused pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogens resistant to them, resulting in a decline in control effectivene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
Inventor 段春华牛赡光刘幸红王清海郑香军周峰田龙军
Owner SHANDONG FOREST SCI RES INST
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