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Method for preparing vaccinium bracteatum thunb leaf pigment through enzymic method

A technology for preparing black rice leaves and enzymatic method, which is applied in the direction of fermentation, can solve the problems of process feasibility limitation, high production cost, unfavorable storage, etc., and achieves the effects of reducing cost input, short production cycle, and facilitating popularization.

Active Publication Date: 2013-12-25
江苏九久环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ceramic membrane purification concentration and freeze-drying technology adopted in this invention has relatively high production costs
When fresh leaves are crushed, they first use their own enzymes to oxidize to produce black rice leaf pigment, but the black rice leaf pigment is extremely heat-resistant, and this characteristic makes the film concentration irreplaceable
In addition, the fresh leaves of black rice tree are not conducive to storage, which limits the feasibility of this process

Method used

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  • Method for preparing vaccinium bracteatum thunb leaf pigment through enzymic method
  • Method for preparing vaccinium bracteatum thunb leaf pigment through enzymic method
  • Method for preparing vaccinium bracteatum thunb leaf pigment through enzymic method

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for enzymatically preparing the leaf pigment of black rice tree, the steps are as follows:

[0021] (1) Enzyme source preparation: crush 5 kg of fresh leaves of the black rice tree, knead and cut them, ultrafinely pulverize them, and use them directly or store them at -16°C~-25°C for later use;

[0022] (2) Precursor solution preparation: select 100kg of fresh leaves of black rice tree in the same year, fry green, and dry at 70°C; take dried black rice leaf leaves and add water, the weight is 8 times the weight of black rice leaves, soak for 5h; at 80°C Extracted twice at high temperature, concentrated under reduced pressure to a density of 1.01g / cm 3 ;

[0023] (3) Enzyme-catalyzed reaction: Add the enzyme source prepared in step (1) to the precursor solution prepared in step (2), and the mass ratio of dry leaves of Azalea japonica contained in the precursor solution: enzyme source mass ratio is 20: 1. React with ventilation and stirring at 20°C for 6 hours ...

Embodiment 2

[0027] A method for enzymatically preparing the leaf pigment of black rice tree, the steps are as follows:

[0028] (1) Enzyme source preparation: crush, knead and cut 20kg of fresh leaves of the black rice tree, and ultrafinely pulverize them, and use them directly or store them at -16°C~-25°C for later use;

[0029] (2) Precursor solution preparation: select 100kg of fresh leaves of the black rice tree that year, steam green, and dry at 90°C; take the dried black rice tree leaves and add water, the weight is 10 times the weight of the black rice tree leaves, soak for 8h; at 65°C Extracted twice at high temperature, concentrated under reduced pressure to a density of 1.05g / cm 3 ;

[0030] (3) Enzyme-catalyzed reaction: Add the enzyme source prepared in step (1) to the precursor solution prepared in step (2), and the mass ratio of dry leaves of A. japonica in the precursor solution: enzyme source mass ratio is 5: 1. React with ventilation and stirring at 37°C for 8 hours to ...

Embodiment 3

[0034] A method for enzymatically preparing the leaf pigment of black rice tree, the steps are as follows:

[0035] (1) Enzyme source preparation: crush, knead and cut 16 g of the fresh leaves of A. sativa, and use them directly or store them at -16°C~-25°C for later use;

[0036] (2) Precursor solution preparation: select 800g of fresh leaves of A. sativa in the same year, bake green, and dry at 40°C; take the dried leaves of A. Extract twice at ℃, concentrate under reduced pressure to a density of 1.08g / cm 3 ;

[0037] (3) Enzyme-catalyzed reaction: Add the enzyme source prepared in step (1) to the precursor solution prepared in step (2), and the mass ratio of dry leaves of A. chinensis contained in the precursor solution: enzyme source mass ratio is 50: 1. React with aeration and stirring at 28°C for 4 hours to obtain the enzymolysis solution; the stirring rate is 100r / min, and the aeration volume is 70L / min;

[0038] (4) Drying: Centrifuge the enzymatic hydrolyzate obta...

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Abstract

The invention relates to a method for preparing vaccinium bracteatum thunb leaf pigment through the enzymic method, and belongs to the technical field of basic solution extraction. The vaccinium bracteatum thunb leaf pigment is prepared through the processes of enzyme source preparing, precursor solution preparing, enzymatic reacting and drying. The oxidase of vaccinium bracteatum thunb leaves is broken in a kill-out mode, and therefore the occurrence that the pigment of the vaccinium bracteatum thunb leaves is produced too early, the pigment is heated and damaged in the process of subsequent extraction and the quality of the pigment of the vaccinium bracteatum thunb leaves is affected can be avoided. According to the enzymatic reaction, fresh vaccinium bracteatum thunb leaf powder is added, the enzyme in the vaccinium bracteatum thunb leaves is directly utilized for oxidation, the enzyme source does not need to be bought additionally, and cost input can be reduced. The method is easy to carry out and short in production cycle, all needed devices are general devices used in modern industry production, and the method can be popularized easily.

Description

technical field [0001] The present invention relates to a method for preparing black rice leaf pigment by enzymatic method, which is specifically a method of preparing black rice leaf pigment by using the enzyme of the fresh leaf itself through biological enzymatic reaction by using the dried black rice leaf as a raw material, belonging to Raw solution extraction technical field. Background technique [0002] Black rice tree, also known as Nanzhu, is a plant of the genus Vaccinium in the Rhododendronaceae family. It is widely distributed in East Asia, including the south of the Yangtze River in China, North Korea, Japan, and Vietnam. It is not distributed in other areas, so foreign research on black rice tree is almost blank. The domestic research has just started. Studies have shown that the leaf pigments of the black rice tree are oxidation (enzymatic oxidation) products of polyphenolic compounds. The pigment can form a blue-black film with protein (such as rice protein,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
Inventor 卢美娟邵京李百健
Owner 江苏九久环境科技有限公司
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