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Enzyme-linked immunosorbent assay (ELISA) detection method for perfluorooctane sulfonate residual in environment

A perfluorooctane sulfonic acid and enzyme-linked immunoassay technology, which is applied in measuring devices, biological testing, material inspection products, etc., can solve the problems of heavy workload, complicated processing process, expensive instruments, etc., and achieve good specificity Effect

Inactive Publication Date: 2014-01-01
SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can meet the requirements of residue analysis, the sample pretreatment process is complicated, the workload is heavy, and the equipment is expensive. The ELISA method has high sensitivity, strong specificity, simple sample pretreatment, convenient operation, and convenient on-site monitoring. Complementary to conventional analytical methods

Method used

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  • Enzyme-linked immunosorbent assay (ELISA) detection method for perfluorooctane sulfonate residual in environment
  • Enzyme-linked immunosorbent assay (ELISA) detection method for perfluorooctane sulfonate residual in environment

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Experimental program
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Effect test

Embodiment 1

[0024] Dilute PFOs standard samples into 7 concentration gradients of 1, 10, 50, 100, 500, 1000, 5000ug / l and blank control, and set carbonate buffer as blank control for serum antibodies. Using the optimal working concentration of the antigen and antibody screened out by the present invention, an indirect competitive ELISA was carried out to prepare a standard curve of antibodies produced by 4 rabbits. Assuming that the OD value of the antibody blank is ODmin, and the OD value of the PFCs blank control is ODmax, the ordinate inhibition rate=[(ODmax-ODmin)-(ODmax-OD to be tested)] / ODmax-ODmin. The logarithm of the PFCs concentration is taken on the abscissa, and a standard curve is drawn according to the corresponding OD492 value, as shown in Figure 1.

[0025] The test results of the standard samples show that there is no difference in the standard curve of the antibody produced by the 4 rabbits, and the curve shows a good linear relationship between 10-1000ug / l, and the slop...

Embodiment 2

[0027] Add 10, 50, 100ug / ml 3 kinds of PFOs standard samples with different concentrations to 4 kinds of water samples with different quality respectively, set up 3 repetitions for each concentration, use distilled water as blank control, and use for enzyme-linked immunoassay method analysis, according to The measured OD value was checked on the standard curve to find the content of PFOs, and the recovery rate was calculated. The results are shown in Attached Table 1.

[0028] Within the linear detection range of the method 10-1000ug / l, the average recovery rate of PFOs was 101.3%, and the average coefficient of variation was 5.93%.

[0029] Supplementary Table 1 The addition recovery rate of different samples under the screening conditions of the present invention

[0030]

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Abstract

The invention relates to an enzyme-linked immunosorbent assay (ELISA) detection method, which can be applied to enzyme-linked immunosorbent assay (ELISA) detection of perfluorooctane sulfonated (PFOs) residual in the environment. The method comprises following steps: step one, synthesizing and identifying PFOs artificial antigen; step two, preparing and purifying PFOs antibody; step three, establishing PFOs indirectly competing ELISA quantitative detection method; step four, substituting PFOs with 9 PFCs with similar structures as that of PFOs, and then respectively measuring the cross-reactivity of the 9 PFCs with an antibody. The technology provides a method of rapidly detecting PFOs residual in the environment, better improves the detection sensitivity, guarantees the precision of the detection results, satisfy the requirements on PFOs residual detection at present, and is convenient for carrying out PFOs residual detection in the environment.

Description

technical field [0001] The invention relates to an enzyme-linked immunological detection method, which can be applied to the enzyme-linked immunological detection of perfluorooctane sulfonic acid residues in the environment. Background technique [0002] Perfluorinated compounds (PFCs) are widely used in many industrial production and daily necessities such as polymer additives and lubricants due to their excellent thermal stability, chemical stability, high surface activity and hydrophobic and oleophobic properties. Perfluorooctane sulfonic acid (Perfluorooctane Sulphonate, PFOs) and perfluorooctanoic acid (Perfluorooctanoic Acid, PFOA) are representative PFCs. In May 2009, the United Nations Environment Program and the Fourth Conference of the Parties to the Stockholm Convention on Persistent Organic Pollutants (POPs) reached a consensus on reducing and finally banning the use of nine toxic chemicals that seriously endanger human health and the natural environment , the n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/543
Inventor 朱江郭小品姚宏伟罗启仕杨洁孟梁朱杰郭琳李忠元张长波李青青王旌高洁朱悦
Owner SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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