A preparation method of fermenting high-temperature soybean meal to produce rich peptide-rich prebiotic feed

A production method and soybean meal technology, applied in animal feed, animal feed, application, etc., can solve the problems of low increase in crude protein content, low palatability, high cost, etc., to achieve improved animal growth performance, improved nutritional characteristics, The effect of significant economic benefits

Active Publication Date: 2016-05-18
JIANGNAN UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many improvements have been made to the process of fermenting soybean meal. For example, the Chinese patent application number 200810219198.7 uses three strains of Bacillus subtilis, Saccharomyces cerevisiae and lactic acid bacteria to ferment soybean meal, which degrades the protein in soybean meal to a certain extent and improves its nutrition. value, but the increase in crude protein content is not high
Liu Xiaoyan and others used Bacillus subtilis, Aspergillus oryzae and Saccharomyces cerevisiae to ferment high-temperature soybean meal, so that the polypeptide yield in the final fermentation product reached 54.89%, and the polypeptide content in the fermentation product was 21.47% (dry basis), which was 4.1 times higher than that of the raw material. Better nutritional value, but the inoculation amount of this method is 25%, the fermentation raw materials need to add bran and molasses to adjust the carbon-nitrogen ratio of the medium, the cost is relatively high, although the flavor of the fermented soybean meal product has improved, it does not have a sour flavor , acidification treatment is still required before feeding, and there is still room for improvement in palatability (Liu Xiaoyan, Yang Guoli, Guo Lidong. Research on the production of soybean polypeptide feed by mixed bacteria solid-state fermentation [J]. Feed Industry, 2012.33(6): 51- 56)
In general, the current high-temperature soybean meal fermentation process still has problems such as high cost, low nutritional value of the product, and low palatability.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of fermenting high-temperature soybean meal to produce rich peptide-rich prebiotic feed
  • A preparation method of fermenting high-temperature soybean meal to produce rich peptide-rich prebiotic feed
  • A preparation method of fermenting high-temperature soybean meal to produce rich peptide-rich prebiotic feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: The example of this specific implementation adopts the following technical scheme: a preparation method for fermenting high-temperature soybean meal to produce peptide-rich prebiotic feed, the preparation steps are: (1) from the original strain Bacillus subtilis, Saccharomyces cerevisiae and Take a loop of Lactobacillus plantarum to beef extract peptone slant medium, YPD slant medium and MRS slant medium respectively, and cultivate at 30℃-37℃ for 18h-24h, until obvious colonies grow on the slant, that is, the bacteria are completed. activation;

[0012] (2) Pick a ring from the activated strains of Bacillus subtilis and Saccharomyces cerevisiae, inoculate it into beef extract peptone liquid medium and YPD liquid medium respectively, and culture at 30℃-37℃ for 18h-48h on a shaking table , until the concentration of microorganisms in the culture solution reaches 106CFU / mL-108CFU / mL, stop the culture, and the seed solution is successfully prepared. Pick a ring ...

Embodiment 2

[0019] The difference from Example 1 is that the aerobic fermentation was performed for 24 hours, and the sealed anaerobic fermentation was performed for 72 hours. The nutritional components of soybean meal before and after fermentation were analyzed as follows:

[0020] Test result 2

[0021]

[0022] Remarks: The contents of the above substances are calculated as dry matter.

Embodiment 3

[0024] The difference from Example 2 is that the initial pH of soybean meal is 6.5, the total inoculation amount of Bacillus subtilis seed solution is 6%, and the inoculum amount of Saccharomyces cerevisiae and Lactobacillus plantarum seed solution is 3%. The nutritional components of soybean meal before and after fermentation were analyzed as follows:

[0025] Test result 3

[0026]

[0027]

[0028] Remarks: The contents of the above substances are calculated as dry matter.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing feed with rich peptide and rich prebiotics by fermenting high-temperature soybean meal, and relates to the technical field of farm products bioprocess. A stain of high-yield protease bacillus subtilis which is sorted from conventional Jiangxi fermented food is used for being matched with brewing yeast and lactobacillus plantarum to carry out the mixed solid fermentation on high-temperature soybean meal, slant activation and liquid enlarge cultivation are carried out, aerobic fermentation is carried out for single inoculation, anaerobic fermentation is carried out for the mixed inoculation, then the high-temperature soybean meal is dried at the temperature of 60 DEG C to obtain a fermented soybean meal feed product in which with protein nutrients are rich, antinutritional factors are passivated almost, the palatability is improved and the bioactivity is high. The method has the advantages that the constitution of the protein and the antinutritional factors of the high-temperature soybean meal fermented product is further improved, the palatability of the fermented product is improved, and the fermented product has the characteristics of rich nutrients, low resistance and palatability.

Description

technical field [0001] The invention relates to the technical field of bioprocessing of agricultural products, in particular to a preparation method of a feed that is rich in peptides and rich in prebiotics produced by fermenting high-temperature soybean meal. Background technique [0002] At present, soybean meal for feed is mainly high-temperature soybean meal. Due to the high-temperature desolvation treatment in the high-temperature soybean meal, the protein in it has a large degree of denaturation and low solubility. At the same time, it also contains some anti-nutritional factors. Absorption is low. Fermented soybean meal is a green and efficient soybean meal processing technology. In recent years, many improvements have been made to the fermented soybean meal process. For example, the Chinese patent application No. 200810219198.7 uses 3 strains of Bacillus subtilis, Saccharomyces cerevisiae and lactic acid bacteria to ferment soybean meal, which degrades the protein i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): A23K10/18
Inventor杨瑞金李舒宇徐张贤金妙仁张燕鹏郑婷婷赵伟
OwnerJIANGNAN UNIV