A screening method for meat preservatives
A screening method and technology of preservatives, applied in the field of screening of meat preservatives, to achieve the effect of improving accuracy and strong selection
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Embodiment 1
[0014] (1) Take the bag to pack the sausage, take the product out in a sterile room, put it into a sterilized meat grinder, and grind it;
[0015] (2) Dissolve nisin with a small amount of sterile water, add it to the pulverizer, and continue stirring for 3 minutes to mix the preservative and the sample evenly, and the dosage of nisin is 0.15g / kg meat filling;
[0016] (3) Put the meat filling into sterilized packaging bags with a medicine spoon, each bag is 100±1g, two bags in total, seal with a vacuum packaging machine, sterilize in a water bath at 90°C for 20 minutes, take out the sample and cool it completely with cold water ;
[0017] (4) Place the cooled sample at 25±1°C for cultivation, and regularly measure and record the number of microorganisms according to the growth of microorganisms until the product is spoiled.
Embodiment 2
[0019] The nisin in the embodiment 1 step (2) is changed into a compound preservative, and the compound preservative consists of 10.0% nisin by weight fraction, 5.5% sodium dehydroacetate, 69.5% gluconic acid-delta-endo It is composed of ester and 15% glucose, the dosage of compound preservative is 2.0g / kg meat stuffing, and other operation steps are the same.
[0020] Among them, the purchaser of nisin used is Liaoning Zhongke Biological Engineering Co., Ltd., the purchaser of sodium dehydroacetate is Qingdao Dawei Biological Engineering Co., Ltd., and the purchaser of glucono-δ-lactone is Anhui Xing Zhou Pharmaceutical Food Co., Ltd.
Embodiment 3
[0022] The nisin in step (2) of Example 1 was changed to potassium sorbate, and the consumption of potassium sorbate was 0.30 g / kg meat stuffing, and other operation steps were the same.
[0023] Table 1 is a comparison table of the experimental record results of Example 1, Example 2 and Example 3.
[0024] Table 1
[0025]
[0026] As can be seen from Table 1: the total number of colonies increases gradually with the prolongation of time, and the total number of colonies of the sample in embodiment 3 grows the fastest, illustrating that the antibacterial effect of potassium sorbate is the worst in three preservatives; embodiment 1 The growth rate of the total number of colonies in the sample is slightly higher than that of the sample in Example 2, indicating that the antibacterial effect of the single product nisin is slightly worse than that of the compound preservative, so the antibacterial effect of the compound preservative is the best among the three preservatives.
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