Animal source feed raw material identification method based on fatty acid detection
An animal-derived feed and fatty acid technology, applied in the field of animal-derived feed identification, can solve problems such as difficult to meet efficient and accurate species identification and analysis, achieve sustainable development and recycling, ensure stability, and meet analysis requirements. desired effect
Active Publication Date: 2015-03-04
CHINA AGRI UNIV
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Problems solved by technology
At present, the standard methods for testing animal-derived feed used in official arbitration in the international scope are mainly microscope analysis and polymerase chain reaction. Certain limitations, single use is difficult to meet the requirements of efficient and accurate species identification analysis
Method used
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Embodiment 1
[0044] 1) Collection and preparation of research samples
[0045] The research samples consisted of 51 animal-derived feeds of known animal species, including products collected from domestic and foreign protein feed enterprises by relevant quality inspection departments and self-made samples. The samples covered terrestrial animal meat meal, bone meal, meat and bone meal , feather meal and fish meal, etc., including 10 samples from pigs, 8 samples from chickens, 9 samples from cattle, 11 samples from sheep and 13 samples from fish).
[0046] All research samples were pulverized by a cyclone mill and passed through a 1.0 mm sieve.
[0047] 2) Sample fatty acid determination
[0048] Using the Soxhlet extraction method, a fully automatic fat analyzer (SoxtecTM2050, FOSS company in Denmark) was used to extract the lipid samples of the above research samples, and then a gas chromatograph (GC-2014C, Japan Shimadzu company) was used to measure common fats with 2-24 carbon atoms. ...
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The invention provides an animal source feed raw material identification method based on fatty acid detection. The identification method comprises the following steps: 1) collecting and preparing different species of animal source feed samples with a known source; 2) detecting fatty acids; 3) determining detected and un-detected information basic data of the fatty acids; 4) establishing a discriminatory analysis model; 5) evaluating the discriminatory analysis model; 6) determining detected and un-detected information data of the fatty acids in the animal source feed samples to be detected, inputting the determined detected and un-detected information data of the fatty acids in the animal source feed samples to be detected in the model and performing species identification analysis. According to the identification method, fatty samples are extracted by using a conventional soxhlet method, so that fatty acid detection results of different species of animal source feeds can be obtained accurately; composition difference characteristics of the fatty acids in different species of animal source feeds are embodied intuitively and significantly; the stability of the identification model is effectively ensured; meanwhile, identification analysis results are displayed intuitively. Through using the identification method, the effective identification analysis of different species of animal source feeds can be realized, so that the analysis requirement of animal source feeds on feed quality and safety supervision can be satisfied.
Description
technical field [0001] The invention belongs to the field of identification of animal-derived feeds, in particular to a method for identification and analysis of different species of animal-derived protein feeds based on fatty acid detection. Background technique [0002] In recent years, the "mad cow disease" incident has brought a huge impact on animal husbandry, public safety, feed industry and international trade of animal products in the EU and even in the world, seriously threatening the health of animals and humans, and causing huge economic losses. In order to cut off the transmission of mad cow disease from the source, countries around the world have enacted laws and regulations to restrict or prohibit the use of animal-derived feed such as meat and bone meal. However, due to the lack of protein feed resources and the drive of interests, the illegal use of animal-derived feeds such as illegal imports, production and sale of fakes, and prohibited additions has been r...
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IPC IPC(8): G01N30/00G01N30/02
Inventor 刘贤蒲乾坤韩鲁佳周兴藩
Owner CHINA AGRI UNIV