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Detection of bacteria having a resistance to carbapenems

A technology of carbapenems and bacteria, applied in the field of detection of bacteria resistant to carbapenems

Inactive Publication Date: 2014-02-19
BIOMERIEUX SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To the applicant's knowledge, they have never been used to detect and / or identify carbapenem-resistant bacterial strains directly in biological samples

Method used

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  • Detection of bacteria having a resistance to carbapenems
  • Detection of bacteria having a resistance to carbapenems
  • Detection of bacteria having a resistance to carbapenems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Testing Faropenem on Pure Strains

[0057] 1. Media and Microorganisms

[0058] 79 strains of Gram-negative bacteria were tested on medium containing different concentrations of faropenem, of which 69 strains were enterobacteria and 10 strains were non-enterobacteria (non-fermenting bacteria), and their resistance characteristics were listed in Table 1, to establish the sensitivity / specificity of each preparation. Plates were read after 24 hours of incubation at 37°C.

[0059] Table I: Characterization of the resistance mechanisms of the tested strains

[0060]

[0061] The medium used was regular agar medium containing at least one chromogenic substrate supplemented with faropenem at the following concentrations:

[0062]

[0063] 2. Test

[0064] Divide the medium into 120X120 square dishes.

[0065] Inoculations were performed from 24-h pre-cultures on trypcase soybean agar at 37 °C.

[0066] For each strain, a 0.5 McF suspension in physiologica...

Embodiment 2

[0079] Doripenem test on the pure strain of embodiment 2

[0080] 1. Media and Microorganisms

[0081] 100 strains of Gram-negative bacteria were tested on media containing different concentrations of faropenem, 98 of which were enterobacteria and 2 strains were non-enterobacteria (non-fermenting bacteria), their resistance characteristics are listed in Table III, to establish the sensitivity and specificity of each preparation. Media containing 0.125 mg / L meropenem were also tested. Chromogenic medium (Biorad) was used as a growth control. Plates were read after incubation at 37°C for 24h and 48h.

[0082] Table III: Characterization of the resistance mechanisms of the tested strains

[0083]

[0084] The medium used was a regular agar medium containing at least one chromogenic substrate supplemented with the following concentrations of carbapenems:

[0085]

[0086] 2. Test

[0087] Divide the medium into 90mm dishes.

[0088] Each strain was spot-inoculated on...

Embodiment 3

[0094] Example 3 Comparison of media containing doripenem with commercially available media

[0095] 1. Media and Microorganisms

[0096] In the medium B (0.065mg / L doripenem) and COLOREX described above T Of the 100 enterobacterial strains tested on MKPC medium, 25 of them produced carbapenemase, and their resistance characteristics are listed in Table V. The latter medium was supplied by BioMed Diagnostics Inc. and corresponds to CHROMagar TM KPC ready-to-use medium.

[0097] Table V: Characterization of the resistance mechanisms of the tested strains

[0098]

[0099] 2. Test

[0100] Use 1 μL (contains about 10 4 microorganisms) diluted 0.5McF suspension to inoculate each strain on each medium.

[0101] Readings were performed after 24 and 48 hours of incubation at 37°C.

[0102] 3. Results and Interpretation

[0103] Table VI: Percentage of strains developed on medium

[0104]

[0105] Media containing 0.065 mg / L doripenem allowed growth of 92% of carbapene...

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Abstract

Disclosed is a process for detecting and / or identifying, in a biological sample, bacteria exhibiting a resistance to carbapenems, including: a) contacting said sample with a reaction medium including at least one chromogenic agent and faropenem and / or doripenem; b) incubating the whole so as to allow the bacteria to grow; and c) detecting the strains exhibiting a resistance to carbapenems. The medium employed in step a) also contains cloxacillin and / or a combination of cloxacillin and PAbetaN.

Description

technical field [0001] The invention relates to a detection and identification method suitable for screening carbapenem-resistant bacteria. Background technique [0002] Increasing resistance to β-lactam antibiotics such as penicillins and cephalosporins complicates the treatment of infections caused by Gram-negative bacterial strains. These antibiotics are therefore replaced by other broad-spectrum antimicrobials. Among these broad-spectrum antimicrobials, carbapenems have played an important role, especially in the treatment of hospitalized patients. Carbapenems act against most Gram-positive and Gram-negative aerobic bacteria and some anaerobic bacteria. [0003] However, carbapenem-resistant strains are increasingly appearing in hospitalized patients. [0004] Relevant bacteria include, but are not limited to, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter sp., Klebsiella pneumoniae ), Klebsiella oxytoca, Pseudomonas aeruginosa, Providenc...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12N1/20
CPCC12N1/20G01N21/763C12Q1/04C12N15/01
Inventor S·吉拉尔迪J·佩里G·赞巴尔迪
Owner BIOMERIEUX SA
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