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Primer, probe and kit for detection of gene expression amounts and use methods thereof

A gene expression and kit technology, applied in the field of molecular biology, can solve the problems of high RNA requirements, low sensitivity, unstable results, etc., and achieve the effects of low price, high detection sensitivity, and simple and fast detection process.

Active Publication Date: 2014-02-26
北京海思特医学检验实验室有限公司
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Problems solved by technology

This method is cumbersome and time-consuming, and has high requirements on RNA, and RNA is very easy to degrade, resulting in unstable results and low sensitivity. The amount of sample RNA required is very large to ensure the detection results

Method used

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  • Primer, probe and kit for detection of gene expression amounts and use methods thereof
  • Primer, probe and kit for detection of gene expression amounts and use methods thereof
  • Primer, probe and kit for detection of gene expression amounts and use methods thereof

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Embodiment Construction

[0050] The specific implementation of the present invention will be further described below in conjunction with the examples, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention.

[0051] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0052] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0053] Example kits for detecting the expression levels of ERCC1, RRM1, TUBB3 and TYMS genes and their use

[0054] 1. The kit for detecting the expression levels of ERCC1, RRM1, TUBB3 and TYMS genes contains:

[0055] (1) Primers and probes for detecting ERCC1, RRM1, TUBB3 and TYMS genes, the primer sequences are shown in SEQ ID NO: 1 ~ SEQ ID NO: 8, and the probe sequen...

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Abstract

The invention discloses a primer, a probe and a kit for detection of gene expression amounts and uses method thereof, and the invention also discloses the kit comprising the primer and the probe and a use method of the kit. The sequences of the primer are shown as SEQ ID NO:1 to SEQ ID NO:8, the sequences of the probe are shown as SEQ ID NO:11 to SEQ IDNO:14 shows, the kit includes the primer, the probe, a primer and a probe of a reference gene, a real-time fluorescent quantitative PCR (polymerase chain reaction) MIX reaction solution, deionized water, bottles or tubes for separated and concentrated packaging of the reagents, and a packing box. The primer, the probe and the kit can detect the expression amounts of four genes, the detection range is wide, the detection process is simple and quick, and the primer, the probe and the kit help a doctor to conduct a comprehensive analysis and judgment of a medication strategy of a patient.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to primers, probes and kits for gene detection, in particular to primers, probes, kits, methods and applications for detecting the expression levels of ERCC1, RRM1, TUBB3 and TYMS genes. Background technique [0002] With the continuous improvement of medical research and drug research and development, more and more drugs for the treatment of tumors have been developed. However, some patients have unsatisfactory effects when using certain drugs for treatment, which is characterized by low tumor sensitivity to drugs. Strong drug resistance, etc. Studies have shown that this phenomenon is related to the overexpression of certain genes in the human body. [0003] Platinum drugs are one of the most commonly used tumor chemotherapy drugs. They mainly form adducts by causing cross-linking of DNA in tumor cells, hindering DNA synthesis and replication, thereby inhibiting the growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 李艳春侯然程宇刘晓峰朱立文梁超王绪华方国伟黄士昂陈忠
Owner 北京海思特医学检验实验室有限公司
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