Preparation method for glabrous greenbrier rhizome extract and use of glabrous greenbrier rhizome extract for skin whitening

A tuckahoe extract and tuckahoe technology are applied in the directions of skin diseases, preparations for skin care, medical preparations containing active ingredients, etc., to achieve good whitening effect, improvement of skin pigmentation, and high safety.

Inactive Publication Date: 2014-03-05
SHANGHAI INOHERB COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In cosmetics, the crude extract of Smilax cocos can be used in whitening compositions; but according to the method described in the present invention, the flavonoid parts and water parts obtained by separating and purifying the crude extract of Smilax cocos have better properties than the original crude extract. The whitening effect, its superior whitening activity and skin whitening application have not been reported

Method used

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  • Preparation method for glabrous greenbrier rhizome extract and use of glabrous greenbrier rhizome extract for skin whitening
  • Preparation method for glabrous greenbrier rhizome extract and use of glabrous greenbrier rhizome extract for skin whitening
  • Preparation method for glabrous greenbrier rhizome extract and use of glabrous greenbrier rhizome extract for skin whitening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Get Smilax tuckahoe to wash, dry, set aside.

[0037] Step 1: Take 25g of Smilax smilax, crush it into a coarse powder (pass No. 2 sieve, no more than 40% can pass No. 4 sieve), extract with 10 times the amount of 80% ethanol, and extract 50% at 90°C. hour, the extract was left to stand, filtered, concentrated, vacuum-dried or freeze-dried until the solvent was completely volatilized, and the crude extract of Smilax tuckahoe was obtained (crude extract A );

[0038] Step 2: After the medicinal extract is dispersed with water, adjust the pH value to 6.0, and use a D101 macroporous adsorption resin column to carry out separation and purification: successively use 3 times the volume of the resin column volume, the volume fraction of 3 times the resin column volume is 50%, and the pH is 8.0 ethanol aqueous solution, 3 times the volume fraction of the resin column volume is 85% ethanol aqueous solution elution, collect the eluent of each position respectively, obtain the el...

Embodiment 2

[0094] Get Smilax tuckahoe to wash, dry, set aside.

[0095] Step 1: Weigh 25g of Smilax smilax, crush it into a coarse powder (pass No. 2 sieve, no more than 40% can pass No. 4 sieve), extract with 20 times the amount of 70% ethanol, and extract 70% at 80°C hour, the extract was left to stand, filtered, concentrated, vacuum-dried or freeze-dried until the solvent was completely volatilized, and the crude extract of Smilax tuckahoe was obtained (extract A );

[0096] Step 2 is identical with embodiment 1, obtains the Smilax tuckahoe extract of each position ( B , C , D ), among them, Smilax tuckahoe extract B , C Be the Smilax tuckahoe extract of the present invention.

Embodiment 3

[0098] Get Smilax tuckahoe to wash, dry, set aside.

[0099] Step 1: Weigh 25g of Smilax smilax, crush it into a coarse powder (no more than 40% can pass through No. 2 sieve), extract with 15 times the amount of 60% methanol, and extract 3 hour, the extract was left to stand, filtered, concentrated, vacuum-dried or freeze-dried until the solvent was completely volatilized, and the crude extract of Smilax tuckahoe was obtained (extract A );

[0100] Step 2: After the extract is dispersed with water, adjust the pH value to 6.0, and use a D101 macroporous adsorption resin column for separation and purification: sequentially use 5 times the volume of the resin column with water, and 5 times the volume of the resin column with an aqueous methanol solution with a volume fraction of 60%. , 5 times the volume fraction of the resin column volume is 85% aqueous methanol elution, the eluent is concentrated, vacuum-dried or freeze-dried until the solvent is completely volatilized, and th...

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Abstract

The invention relates to a preparation method for glabrous greenbrier rhizome extract. The method comprises the following steps of adding coarse powder into an organic solvent-aqueous solution, performing refluxing extraction, and performing standing, filtering and concentration to obtain glabrous greenbrier rhizome crude extract; dispersing the extract by using water, performing separation and purification by using a macroporous adsorption resin column, performing elution sequentially by using water, a 40 to 80 percent organic solvent-aqueous solution and an 85 to 95 percent organic solvent-aqueous solution, and concentrating and drying eluent of each part to obtain extract of each part, wherein the glabrous greenbrier rhizome extract is obtained by performing concentration and drying on the part eluted by water or the 40 to 80 percent organic solvent-aqueous solution, and is used for inhibiting the activity of tyrosinase and the synthesis of melanin, and a composition comprises 0.01 to 15 mass percent of glabrous greenbrier rhizome extract. The preparation method has the advantages that various whitening effect tests show that the glabrous greenbrier rhizome extract can be used for effectively inhibiting the activity of the tyrosinase and the synthesis of the melanin, can be used for an external skin product as an active whitening raw material, and is high in safety, and the effects of improving skin pigmentation and whitening skin can be achieved.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, especially a preparation method of the smilax smilax extract, and the use of the extract as an active ingredient in skin whitening. Background technique [0002] Melanin is a high-molecular-weight biopigment secreted by melanocytes, which exists in the middle of the cells in the basal layer of the skin. When the skin is exposed to ultraviolet rays, a large amount of melanin is produced, and melanin can absorb ultraviolet rays to protect the skin. There are many reasons for the formation of melanin, and ultraviolet radiation is only one of the important reasons. The difference in human skin color mainly depends on the content of melanin in the skin and melanosomes in the mature stage. Inhibiting the synthesis of melanin can effectively improve the color of the skin and achieve a whitening effect. [0003] Tyrosinase is the key enzyme in the synthesis of melanin, and it is an essential su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K36/90A61K36/896A61P17/00A61Q19/02
Inventor 雷杨符移才张彬锋王鞠骏白晓云胡国胜
Owner SHANGHAI INOHERB COSMETIC
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