Method for extracting proteins in chicken foot skin through alkaline process
A chicken feet skin and alkali extraction technology, which is applied in the field of agricultural and sideline product utilization, can solve the problems of limited knowledge of chicken feet skin and less research, and achieves the effects of improving processing and utilization efficiency and reducing environmental pollution.
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Embodiment 1
[0006] The chicken feet skin is pulverized, passed through a 30-mesh sieve to obtain the chicken feet skin powder, and degreased with n-hexane to obtain the defatted chicken feet skin powder. Accurately weigh 30g of chicken feet skin degreasing raw materials, add 1000mL of 1.5% sodium hydroxide solution, heat in a water bath at 70°C and stir continuously for 7 hours, centrifuge at 3500r / min for 15min, take the supernatant and dehydrate to obtain chicken feet Skin protein products, the extraction rate of chicken feet skin protein is 84.2%.
Embodiment 2
[0008] The chicken feet skin is pulverized, passed through a 30-mesh sieve to obtain the chicken feet skin powder, and degreased with n-hexane to obtain the defatted chicken feet skin powder. Accurately weigh 30g of chicken feet skin degreasing raw materials, add 1200mL of 1.5% sodium hydroxide solution, heat in a water bath at 60°C with constant stirring for 5 hours, centrifuge at 3500r / min for 15min, take the supernatant and dehydrate to obtain chicken feet Skin protein products, the extraction rate of chicken feet skin protein is 83.6%.
Embodiment 3
[0010] The chicken feet skin is pulverized, passed through a 30-mesh sieve to obtain the chicken feet skin powder, and degreased with n-hexane to obtain the defatted chicken feet skin powder. Accurately weigh 30g of chicken feet skin degreasing raw materials, add 1000mL of 1.5% sodium hydroxide solution, heat in a water bath at 65°C and stir continuously for 6 hours, centrifuge at 3500r / min for 15min, take the supernatant and dehydrate to obtain chicken feet The skin protein product, the extraction rate of chicken foot skin protein is 84.1%.
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