Method for extracting proteins from outer skin of chicken feet

Abstract

The invention relates to a method for extracting proteins from outer skin of chicken feet, belonging to the technical field of utilization of agricultural and sideline products. The method comprises the following steps of taking the outer skin of the chicken feet as a raw material, crushing, and screening by a 30-mesh screen to obtain powder of the outer skin of the chicken feet; degreasing with n-hexane to obtain the degreased powder of the outer skin of the chicken feet; digesting the degreased powder of the outer skin of the chicken feet at normal pressure for 2h, and dehydrating and drying to obtain the digested and degreased powder of the outer skin of the chicken feet; then accurately weighing 30g of the powder, adding 1000-1200ml of buffer solution with the pH value of 8, heating to 46 DEG C, adding 3000U-8000U of trypsin, stirring and reacting for 5-8h, performing enzyme inactivation treatment, then centrifuging for 15min at the speed of 3500r / min, taking supernatant liquid, and dehydrating to obtain a protein product of the outer skin of the chicken feet. The extraction rate of the proteins from the outer skin of the chicken feet achieves 76%.

Description

technical field [0001] The invention discloses a method for extracting protein from the skin of chicken feet, which belongs to the technical field of utilization of agricultural by-products. technical background [0002] According to the forecast of FAPRI of the United States, by 2018, the global broiler chicken production will be close to 80 million tons, and the annual growth rate will still be 2% in the future, while China may be higher than the world, reaching 2.5%. In 2011, my country's broiler chicken output was 13.2 million tons, accounting for about 17% of the total meat, an increase of 5% over 2010. In 2012, the output reached more than 13.8 million tons. The skin of chicken feet is different from the skin of mammals. During the processing of chicken feet, the skin can be removed by scalding with hot water. Get about 100,000 tons of chicken foot skins in the world every year. Long-term discarded accumulation will cause great harm to the ecological environment, pose...

AI Technical Summary

Problems solved by technology

By searching domestic and foreign literature in the past ten years, there are almost no research reports on the deep development and utilization of chicken feet peeling, and scientists in related fields have not received due attention. There are very few related studies, so the current understanding of chicken feet skin is very limited