Fermentation method of welan gum

A fermentation method and the technology of welan gum, applied in the field of welan gum fermentation, can solve the problems of no commercial production, etc., and achieve the effects of being beneficial to anabolism, stable properties and improving utilization rate

Active Publication Date: 2014-03-05
GUANGDONG ZHONGKE TIANYUAN NEW ENERGY SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, only CP Kelco in the United States has realized the commercial production of Welan Gum, and there is no report of commercial production in China

Method used

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  • Fermentation method of welan gum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Step 1, preparing hydrolyzed sugar liquid: pretreating cornstarch and enzymatically hydrolyzing it to obtain hydrolyzed sugar liquid with a glucose mass concentration of 3%;

[0047] Step 2, preparation of fermentation medium: take 6L of the hydrolyzed sugar solution obtained in step 1 as a carbon source, and add 0.35% (mass) peptone, 0.2% (mass) K 2 HPO 4, 0.015% (mass) MgSO 4 , 0.2‰ (quality) bubble enemy, make fermentation medium, initial pH7.2;

[0048] Step 3, prepare seed solution: inoculate the activated welan gum production strain ATCC31555 into the seed medium, and cultivate at 28°C for 16 hours to obtain the seed solution; wherein the seed medium (mass concentration %): glucose 1%, peptone 1% , yeast powder 0.50%, K 2 HPO 4 0.2%, MgSO 4 0.01%, initial pH6.5;

[0049] Step 4, Welan Gum Fermentation: Inoculate the seed solution obtained in Step 3 into a 10L fermenter equipped with 6L of the fermentation medium obtained in Step 2 to ferment to obtain a ferm...

Embodiment 2

[0052] Step 1, preparing hydrolyzed sugar liquid: pretreating cornstarch and enzymatically hydrolyzing it to obtain hydrolyzed sugar liquid with a glucose content of 3%;

[0053] Step 2, preparation of fermentation medium: take 6L of the hydrolyzed sugar solution obtained in step 1 as a carbon source, and add 0.35% (mass) peptone, 0.2% (mass) K 2 HPO 4 , 0.15‰ (mass) MgSO 4 , 1‰ (quality) Tween-80, 0.2‰ (quality) soaked in enemy to make fermentation medium, initial pH7.2;

[0054] Step 3, preparation of seed solution: inoculate the activated welan gum production strain ATCC31555 into the seed medium, and cultivate at 30°C for 15 hours to obtain the seed solution; wherein the seed medium (mass concentration %): glucose 1%, peptone 1% , yeast powder 0.5%, K 2 HPO 4 0.2%, MgSO 4 0.01%, initial pH7.2;

[0055] Step 4, welan gum fermentation: inoculate the seed solution obtained in step 3 into a 10L fermenter equipped with 6L of the fermentation medium obtained in step 2 to f...

Embodiment 3

[0064] Step 1, preparing hydrolyzed sugar solution: pretreating and enzymatically hydrolyzing tapioca starch to obtain cassava hydrolyzed sugar solution with a glucose content of 4%;

[0065] Step 2, preparation of fermentation medium: take 7L of the hydrolyzed sugar solution obtained in step 1 as a carbon source, and add 0.4% (quality) beef extract, 0.25% (quality) K 2 HPO 4 , 0.1% (mass) MgSO 4 , 0.5 ‰ (quality) sodium dodecylsulfonate and 0.2 (quality) ‰ foam enemy made fermentation medium, initial pH 7.0;

[0066] Step 3, preparation of seed solution: inoculate the activated welan gum production strain ATCC31555 into the seed medium, and cultivate at 32°C for 14 hours to obtain the seed solution; wherein the seed medium (mass concentration %): glucose 1%, peptone 1% , yeast powder 0.5%, K 2 HPO 4 0.2%, MgSO 4 0.01%, initial pH7.5;

[0067] Step 4, Welan Gum Fermentation: Inoculate the seed liquid obtained in Step 3 into the fermentation medium for fermentation to obt...

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Abstract

The invention discloses a fermentation method of welan gum. The method comprises the following steps of: subjecting starch or cellulose type raw materials to pretreatment and enzyme hydrolysis to obtain a hydrolysed sugar solution; preparing a fermentation culture medium by using the hydrolysed sugar solution as a carbon source and by adding nitrogen sources, inorganic salts and surfactants; inoculating a preserved strain used for producing the welan gum into a seed culture medium and culturing to obtain a seed solution; inoculating the seed solution to the fermentation culture medium and fermenting to obtain a fermentation liquid; and subjecting the fermentation liquid to extraction, drying, smashing and sieving processes to obtain the welan gum. Certain amounts of the surfactants are added into the fermentation culture medium. By a manner of supplementing materials and adopting different stirring speeds in different stages in the fermentation process, dissolved oxygen in the fermentation culture medium and the conversion rate of glucose by the strain are increased, an objective of energy conservation and consumption reduction is achieved, and the comprehensive utilization rate of the starch and the cellulose type raw materials are effectively increased.

Description

technical field [0001] The invention belongs to the field of welan gum production, and in particular relates to a method for fermenting welan gum. Background technique [0002] Starch raw materials include corn starch, potato starch, tapioca starch, etc., which can be used in food, papermaking, energy and other fields. Cellulosic raw materials are generally roots, stems, and leaves of plants, including straw, etc., and their main components are cellulose, hemicellulose, and lignin. Among them, cellulose and hemicellulose are the source of fermentable sugar, and the content accounts for 66-75% of the wood fiber (the dry weight of the cellulosic raw material). Starch and cellulose raw materials exist in large quantities in nature and can be continuously regenerated, and people pay more and more attention to their utilization. [0003] At present, the use of cellulose raw materials is mainly to produce fuel ethanol, but the cost of cellulose hydrolysis is too high (mainly the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
Inventor 梁锏文江宏文李智斌陈柏铨唐兆兴姜新春周宏才
Owner GUANGDONG ZHONGKE TIANYUAN NEW ENERGY SCI & TECH
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