Fermentation method of welan gum

Abstract

The invention discloses a fermentation method of welan gum. The method comprises the following steps of: subjecting starch or cellulose type raw materials to pretreatment and enzyme hydrolysis to obtain a hydrolysed sugar solution; preparing a fermentation culture medium by using the hydrolysed sugar solution as a carbon source and by adding nitrogen sources, inorganic salts and surfactants; inoculating a preserved strain used for producing the welan gum into a seed culture medium and culturing to obtain a seed solution; inoculating the seed solution to the fermentation culture medium and fermenting to obtain a fermentation liquid; and subjecting the fermentation liquid to extraction, drying, smashing and sieving processes to obtain the welan gum. Certain amounts of the surfactants are added into the fermentation culture medium. By a manner of supplementing materials and adopting different stirring speeds in different stages in the fermentation process, dissolved oxygen in the fermentation culture medium and the conversion rate of glucose by the strain are increased, an objective of energy conservation and consumption reduction is achieved, and the comprehensive utilization rate of the starch and the cellulose type raw materials are effectively increased.

Description

technical field

[0001] The invention belongs to the field of welan gum production, and in particular relates to a method for fermenting welan gum. Background technique

[0002] Starch raw materials include corn starch, potato starch, tapioca starch, etc., which can be used in food, papermaking, energy and other fields. Cellulosic raw materials are generally roots, stems, and leaves of plants, including straw, etc., and their main components are cellulose, hemicellulose, and lignin. Among them, cellulose and hemicellulose are the source of fermentable sugar, and the content accounts for 66-75% of the wood fiber (the dry weight of the cellulosic raw material). Starch and cellulose raw materials exist in large quantities in nature and can be continuously regenerated, and people pay more and more attention to their utilization.

[0003] At present, the use of cellulose raw materials is mainly to produce fuel ethanol, but the cost of cellulose hydrolysis is too high (mainly the...

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