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Compound biological preservative and application method thereof

A biological antiseptic and preservative technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of excessive pesticide and organic compound residues, restrictions on the type and dosage of fungicides, and the general effect of fruit and vegetable disease control, etc. problems, achieve broad-spectrum antibacterial and disease prevention effects, no pesticide residues, and good compatibility

Active Publication Date: 2015-04-22
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of chemical pesticides and antibiotics to stored fruits and vegetables faces the following three problems: First, the application standards of fungicides for harvested fruits and vegetables are relatively high, and the types and dosages of fungicides that can be applied are strictly limited. The widespread use of post-harvest vegetables, the residues of pesticides and organic compounds seriously exceed the standard; in addition, the resistance of post-harvest pathogens is increasing, and the cost of developing new high-efficiency fungicides that meet current market standards is high, and there are not many alternative fungicides to choose from ;Finally, with the growing awareness of the environment and food safety, people think that chemical fungicides and antibiotics are harmful to human health and threaten the safety of the environment. International trade barriers to protect the country's fruit and vegetable industry
However, generally an antagonistic bacteria has a relatively single antibacterial spectrum, and its effect on the control of postharvest vegetable diseases is average, compared with chemical fungicides. How to achieve biological control based on microorganisms without harming human health? Threat to environmental safety is a common problem in current biological control technology

Method used

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  • Compound biological preservative and application method thereof
  • Compound biological preservative and application method thereof
  • Compound biological preservative and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the influence of natamycin on the growth of Bacillus amyloliquefaciens BGP20

[0033] 1) Preparation of seed liquid: take the glycerol-preserved bacterial strain of Bacillus amyloliquefaciens BGP20, inoculate it on a 2YT solid plate by streaking, activate and culture at 30°C for 36 h, and pick a single colony on a 25 mL 2YT liquid medium in a 100 mL Erlenmeyer flask, cultured for 36 h at a shaking frequency of 150 r / min, 30 °C, as a seed solution.

[0034] 2) Preparation of 2YT liquid medium containing natamycin: use sterilized water to prepare a mixed solution containing 5% natamycin as the mother solution, and sterilize at 85°C for 20 min. Add the sterilized natamycin stock solution into a 100 mL Erlenmeyer flask containing 25 mL of sterilized 2YT liquid medium, and the final concentrations of natamycin are 0.05% (m / v), 0.1% (m / v), 0.5% (m / v).

[0035] 3) Growth measurement: Inoculate the seed bacteria solution into 2YT medium with different natamycin...

Embodiment 2

[0037] Embodiment 2, the impact of natamycin on the germination rate of BGP20 spores

[0038] Transfer the BGP20 seed liquid to 2YT medium at a ratio of 1% (v / v), and culture at 30°C and 150 r / min for 72 h. Add natamycin to the liquid 2YT agar medium, the final concentrations of natamycin are 0.1% (m / L), 0.5% (m / L) and 1.0% (m / L) respectively, pour the plate for later use . The fermentation broth was placed in a water bath at 80°C for 20 min, diluted with sterilized distilled water, and spread on 2YT plates containing different concentrations of natamycin. 2YT agar plate without natamycin was used as control. Then culture them upside down at 30°C for 24 h, and investigate the number of colonies on each plate. Three plates were coated for each concentration gradient, and the same experiment was performed twice.

[0039] Such as image 3 As shown, it can be seen from the results that natamycin has no effect on the germination rate of BGP20 spores, even if the concentration ...

Embodiment 3

[0040] Embodiment 3, the impact of natamycin on the stability of BGP20 fermented liquid biocontrol agent

[0041] The BGP20 seed broth was transferred to the fermentation medium at a ratio of 1% (v / v), and cultured at 30°C and 150 r / min for 72 h. Natamycin was added to the BGP20 fermentation broth to make the final concentrations 0.1% (m / v) and 0.5% (m / v), respectively, and the BGP20 fermentation broth without natamycin was used as a control. The bacterial solutions of each treatment were stored at room temperature, and the number of viable bacteria in the cultured bacterial solutions of each treatment was investigated at 0 d, 30 d, and 60 d after treatment, and colony counts were carried out by using the method of gradient dilution plate coating. In addition, 3 sterilized test tubes were filled with each treated bacterial liquid, and each test tube was filled with 10 mL of bacterial liquid, left at room temperature, and the bacterial settlement was continuously observed, and ...

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Abstract

The invention discloses a compound biological preservative. The compound biological preservative comprises the components as follows: 0.1-5 g of component B is added in each 1L of component A, the component A is fermentation liquor of Bacillusamyloliquefacienssubsp.plantarum BGP20 strains, and the component B is natamycin. The compound biological preservative is used for preventing and controlling diseases of fruits and vegetables in a storage period and particularly can be used for simultaneously preventing and treating bacterial soft rot and various fungal diseases (for example, phytophthora capsici, rhizopus soft rot, pythium blight and the like) of fruits and vegetables in the storage period, avoiding or reducing residual chemical pesticides and agricultural antibiotics and maintaining the quality of the fruits and vegetables in the storage period, so that the compound biological preservative has good economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of storage and preservation of agricultural products, and in particular relates to a composite biological antiseptic and antistaling agent composed of Bacillus amyloliquefaciens BGP20 and natamycin and its application, which is specially used for bacterial soft rot and mold diseases of fruits and vegetables during storage pollution-free biological control. Background technique [0002] Infection of pathogenic microorganisms is the main cause of spoilage of harvested fruits and vegetables. In developing countries, the losses caused by it generally account for 20% to 25% of the fruit and vegetable output, and in severe cases, it can reach more than 50%. Various weakly parasitic or epiphytic molds, such as Rhizopus ( Rhizopus sp.), Penicillium ( Penicillium sp.), Pythium ( Python sp.), Phytophthora ( Phytophthora sp.), etc., are the main pathogenic fungi that cause spoilage of harvested vegetables. E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B7/155C12N1/20C12R1/07
Inventor 赵延存黄开红李鹏霞李建军马艳弘
Owner JIANGSU ACAD OF AGRI SCI
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