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High flux quantitative analysis method for visible components in human or animal excretion

A technology for quantitative analysis and excretion, applied in the field of medical inspection, can solve problems such as the inability to standardize the operation process, unstable test results, hidden dangers of biological safety, etc., and achieve the effect of being beneficial to biological protection, meeting the requirements of biological safety, and being highly representative.

Active Publication Date: 2014-03-19
HUNAN OUJIE BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above collection, inspection and analysis process of human or animal secretions has the disadvantages of biological safety hazards, unhygienic operation, strong odor, etc. The test results are unstable, and it is difficult to reproduce the same test results repeatedly. The data has a large deviation, and the operation process cannot be standardized

Method used

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  • High flux quantitative analysis method for visible components in human or animal excretion
  • High flux quantitative analysis method for visible components in human or animal excretion
  • High flux quantitative analysis method for visible components in human or animal excretion

Examples

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Embodiment 1

[0030] Taking human excretion as an example, the method of the present invention will be further described by using the measurement method of the CCD scanning sensor.

[0031] (1) Take a sampler such as figure 1 As shown, the sampler is a sampling rod 1 with blades 2 at the lower end. There are multiple blades 2 at the lower end of the sampling rod 1, which are connected together at the center and are shaped like windmill blades, and are spaced apart from each other to form a fixed-sized space area. ; A central hole 3 is provided in the middle of the sampling rod for adding diluent, and the central hole 3 is provided with multiple outlets at the junction of the blades, and the outlets are evenly distributed. The sampling stick 1 is inserted into human excretion, the sampling stick 1 is rotated, and the excretion is taken into the area between the leaves. Since the area between the blades 2 at the lower end of the sampling rod 1 is fixed and calculated during design, the sampl...

Embodiment 2

[0039] The method of the present invention will be further elaborated below in the manner of weighing and measuring.

[0040] (1) Use a limited sampler to collect human secretions as samples to be tested.

[0041] (2) Take a container with a weight of G1, and add the sample to be tested and the diluent with a weight of G2 into the container.

[0042] (3) Stir the diluent and the sample to be tested evenly to form a suspension. The stirring method is ordinary manual stirring. Put a stirring rod into the container and stir continuously for about 5 minutes, so that the diluent and the sample to be tested are fully mixed evenly to form a suspension.

[0043](4) Accurately weigh the container containing the suspension with an analytical balance of 1 / 100,000, assuming that the weight to be weighed is G3. From this, the weight of the sample to be tested in the container can be calculated as G4=G3-G2-G1, thereby calculating the concentration of the suspension.

[0044] (5) Take a t...

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Abstract

The invention relates to a high flux quantitative analysis method for visible components in human or animal excretion, which comprises the following steps: sampling with limitation, quantitative dilution, stirring, measurement, injection into counting cell, image acquisition and analysis, etc. The invention has the advantages that the method can be used for quantitative analysis and can precisely count number of visible components in per volume or weight of human or animal excretion with accurate result, thereby avoiding uncertainty in traditional sampling analysis method.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a high-throughput quantitative analysis method for formed components in human or animal secretions. Background technique [0002] At present, the collection, inspection and analysis process of human or animal excrement is as follows: take a part of the excretion and put it in a container, such as a carton or a plastic cup, the collector will send the container to the inspection room, and the inspector will dilute 2 drops Drop the liquid on the glass slide, and then use a cotton swab or a small bamboo piece to take a mung bean-sized sample from the container and apply it on the glass slide, stir it evenly, and then observe it under a microscope. At the same time, observe the physical properties of the sample such as color, hardness, mucus, etc., use a low-power lens to observe the number of eggs, such as the number of protozoa, hookworms, roundworm eggs, and then use a high-power le...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N1/38
Inventor 凌铁如张之曦李志雄湛璇谢文锋
Owner HUNAN OUJIE BIOTECH DEV
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