High flux quantitative analysis method for visible components in human or animal excretion

A technology for quantitative analysis and excretion, applied in the field of medical inspection, can solve problems such as the inability to standardize the operation process, unstable test results, hidden dangers of biological safety, etc., and achieve the effect of being beneficial to biological protection, meeting the requirements of biological safety, and being highly representative.

Active Publication Date: 2014-03-19
HUNAN OUJIE BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above collection, inspection and analysis process of human or animal secretions has the disadvantages of biological safety hazards, unhygienic operation, strong odor, etc. T

Method used

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  • High flux quantitative analysis method for visible components in human or animal excretion
  • High flux quantitative analysis method for visible components in human or animal excretion
  • High flux quantitative analysis method for visible components in human or animal excretion

Examples

Experimental program
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Example Embodiment

[0029] Example 1

[0030] In the following, human excretion is used as an example, and the method of the present invention is further explained using the measurement method of a CCD scanning sensor.

[0031] (1) Take a sampler, such as figure 1 As shown, the sampler is a sampling rod 1 with blades 2 at the lower end. There are multiple blades 2 at the lower end of the sampling rod 1, which are connected together at the center to form a windmill blade shape with a space area of ​​a fixed size. The middle of the sampling rod is provided with a central hole 3 for adding diluent, and the central hole 3 is provided with multiple outlets at the intersection of the blades, and the outlets are evenly distributed. Insert the sampling rod 1 into the human excrement, rotate the sampling rod 1, and the excrement is collected into the area between the leaves. Since the area between the blades 2 at the lower end of the sampling rod 1 is fixed and calculated during design, the sample to be insp...

Example Embodiment

[0038] Example 2

[0039] In the following, the method of the present invention will be further explained in the manner of weighing and measurement.

[0040] (1) Use a limited sampler to collect human excreta as samples to be tested.

[0041] (2) Take a container with a weight of G1, and add the sample to be tested and the diluent with a weight of G2 into the container.

[0042] (3) Stir the diluent and the sample to be tested evenly to form a suspension. The stirring method is ordinary manual stirring. The stirring rod is put into the container and stirred continuously, and the stirring time is about 5 minutes, so that the diluent and the sample to be tested are fully mixed uniformly to form a suspension.

[0043] (4) Use a one hundred thousandth analytical balance to accurately weigh the container with the suspension, assuming the weighed weight G3. From this, the weight of the sample to be tested in the container can be calculated as G4=G3-G2-G1, thereby calculating the concentra...

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Abstract

The invention relates to a high flux quantitative analysis method for visible components in human or animal excretion, which comprises the following steps: sampling with limitation, quantitative dilution, stirring, measurement, injection into counting cell, image acquisition and analysis, etc. The invention has the advantages that the method can be used for quantitative analysis and can precisely count number of visible components in per volume or weight of human or animal excretion with accurate result, thereby avoiding uncertainty in traditional sampling analysis method.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a high-throughput quantitative analysis method for formed components in human or animal secretions. Background technique [0002] At present, the collection, inspection and analysis process of human or animal excrement is as follows: take a part of the excretion and put it in a container, such as a carton or a plastic cup, the collector will send the container to the inspection room, and the inspector will dilute 2 drops Drop the liquid on the glass slide, and then use a cotton swab or a small bamboo piece to take a mung bean-sized sample from the container and apply it on the glass slide, stir it evenly, and then observe it under a microscope. At the same time, observe the physical properties of the sample such as color, hardness, mucus, etc., use a low-power lens to observe the number of eggs, such as the number of protozoa, hookworms, roundworm eggs, and then use a high-power le...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N1/38
Inventor 凌铁如张之曦李志雄湛璇谢文锋
Owner HUNAN OUJIE BIOTECH DEV
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