The invention provides a test simulation instrument of an automotive air conditioning controller. The test simulation instrument is used for connecting a computer and the conditioning controller respectively and carrying out simulation test operation. The test simulation instrument of the automotive air conditioning controller at least comprises a universal serial bus (USB) communication module, a K-line communication module, a controller area network (CAN) bus communication module, an analogue-digital (AD) sampling module, a digital signal input module, an analogue output simulation module, a digital output simulation module, an air conditioning power supply module and a central processing module. The test simulation instrument is used for monitoring various input signal states, and simulating and outputting an external signal which is required by the controller simultaneously; the test simulation instrument is connected with the computer through a USB interface and displays a current state of each piece of equipment and a simulation output signal on the computer intuitively, so operation is simple, large-scale production test and product development application can be carried out conveniently, and high intelligence is further realized; and the test simulation instrument is characterized by high accuracy, convenient operation, high efficiency, cost saving and the like, so research and development capacity and production capacity are improved remarkably.