Detection method of sweet potato black rot pathogen

A black spot fungus and detection method technology, applied in the field of bioengineering, can solve the problems of time-consuming and labor-intensive, incapable of rapid disease detection, and achieve the effect of preventing misdiagnosis and preventing spread
CN103667494AActive Publication Date: 2014-03-26INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
Publication Date
2014-03-26

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Abstract

The invention relates to a detection method of sweet potato black rot pathogen, and belongs to the technical field of bioengineering. The method specifically comprises steps as follows: a pair of sweet potato black rot pathogen specific primers SPCPF and SPCPR is designed and synthesized; total DNA (deoxyribonucleic acid) of a to-be-tested sample is extracted and taken as a template, and the primers SPCPF and SPCPR are used for performing PCR (polymerase chain reaction) amplification; and an amplification product is detected through agarose gel electrophoresis. The method is simple, convenient, fast and sensitive, not only can be used for detecting pathogen mycelia but also can be used for detecting sick potato seedlings and pieces; the detection method can be used for performing early detection on sweet potato black rot and further can be used for effectively distinguishing similar diseases, such as a black nevus disease, soft rot and dry rot, of sweet potatoes; and the method has significance on early warning and propagation prevention and control of the sweet potato black rot pathogen.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering and relates to a detection method for pathogens, in particular to a detection method for sweet potato black spot pathogen. Background technique

[0002] Sweet potato black spot disease is caused by the fungus C. Ceratocystis fimbriata Ellis and Halsted) is one of the three major diseases that seriously endanger sweet potato production. The annual yield loss due to this disease is generally 5% to 10%, and the loss caused by the disease is as high as 20% to 50%. or even higher. In addition, diseased potatoes can also produce toxic substances such as ipomeama rone and ipomeanine, which can cause poisoning and even death to humans and livestock after eating. The genus Ascomycota, Sclerotinia, Spheroidales, Sclerosaceae, Sclerosaceae. Infection of sweet potato mainly invades from wounds, and can also invade from bud eyes, lenticels, and root eyes on potato pieces.

[0003] Sweet potato blac...

Claims

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