Preparation method of multilayer PEG-DMA hydrogel

A PEG-DMA and hydrogel technology, which is applied in the field of PEG-DMA hydrogel preparation, can solve problems such as detachment, instability, and poor mechanical strength, and achieve good softness, avoid deformation, and easy operation

Active Publication Date: 2014-04-09
GUANGDONG XIAN JIAOTONG UNIV ACADEMY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the PEG-DMA hydrogels used in tissue engineering are all single-layer and isotropic, however, biological tissues (such as skin, etc.) are usually multi-layer and anisotropic, and the existing single-layer PEG -DMA hydrogels are difficult to meet the anisotropic requirements of biological tissues
However, since PEG-DMA hydrogel is a kind of water-containing biosoft material, it is soft, not easy to adhere, and has poor mechanical strength, so it is very easy to damage, deform, and damage between layers when building a multilayer structure. It is difficult to construct a stable multi-layer PEG-DMA hydrogel structure due to falling off and unstable combination

Method used

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  • Preparation method of multilayer PEG-DMA hydrogel
  • Preparation method of multilayer PEG-DMA hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of double-layer PEG-DMA hydrogel

[0034] Take two glass clips, a U-shaped silicone rubber strip with a thickness of 2mm and several fixing clips. Place the U-shaped silicone rubber strip between the two glass clips, and then use the fixing clip to connect the glass clip to the silicone rubber. The strips are clamped to make a U-shaped single-layer mold with a thickness of 2 mm.

[0035] Add 20mL deionized water and 2g PEG-DMA to the beaker, stir evenly to obtain a 10% mass concentration PEG-DMA solution; then add 0.1mL 2-hydroxy-2-methylpropiophenone and wrap the beaker with tin foil to avoid Stir lightly for more than 30 minutes to prepare the first layer of PEG-DMA solution; use a syringe to inject the first layer of PEG-DMA solution into a U-shaped single-layer mold, and then place it in a UV crosslinker for light crosslinking for 2 minutes to obtain The first layer of PEG-DMA hydrogel with a mass concentration of 10% and a thickness of 2mm.

[003...

Embodiment 2

[0039] Example 2: Preparation of double-layer PEG-DMA hydrogel

[0040] Take two glass clips, a U-shaped silicone rubber strip with a thickness of 2mm and several fixing clips. Place the U-shaped silicone rubber strip between the two glass clips, and then use the fixing clip to connect the glass clip to the silicone rubber. The strips are clamped to make a U-shaped single-layer mold with a thickness of 2 mm.

[0041] Add 20mL deionized water and 2g PEG-DMA to the beaker, stir evenly to obtain a 10% mass concentration PEG-DMA solution; then add 0.1mL 2-hydroxy-2-methylpropiophenone and wrap the beaker with tin foil to avoid Stir lightly for more than 30 minutes to prepare the first layer of PEG-DMA solution; use a syringe to inject the first layer of PEG-DMA solution into a U-shaped single-layer mold, and then place it in a UV crosslinker for light crosslinking for 2 minutes to obtain The first layer of PEG-DMA hydrogel with a mass concentration of 10% and a thickness of 2mm.

[004...

Embodiment 3

[0045] Example 3: Preparation of double-layer PEG-DMA hydrogel

[0046] Take two glass clips, a U-shaped silicone rubber strip with a thickness of 4mm and several fixing clips. Place the U-shaped silicone rubber strip between the two glass clips, and then use the fixing clip to connect the glass clip to the silicone rubber. The strips are clamped to make a U-shaped single-layer mold with a thickness of 4 mm.

[0047] Add 20mL of deionized water and 3g of PEG-DMA to the beaker and stir well to obtain a PEG-DMA solution with a mass concentration of 15%; then add 0.1mL of 2-hydroxy-2-methylpropiophenone and wrap the beaker with tin foil to avoid Stir lightly for more than 30 minutes to prepare the first layer of PEG-DMA solution; use a syringe to inject the first layer of PEG-DMA solution into a U-shaped single-layer mold, and then place it in a UV crosslinker for light crosslinking for 2 minutes to obtain The first layer of PEG-DMA hydrogel with a mass concentration of 15% and a thi...

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Abstract

The invention discloses a preparation method of a multilayer PEG-DMA hydrogel. The preparation method comprises the following steps: 1) taking PEG-DMA, and adding into deionized water to uniformly stir to prepare a first-layer PEG-DMA solution; (2) adding an evocating agent into the first-layer PEG-DMA solution prepared in the step (1), uniformly stirring while keeping out of the sun, feeding into a mold, and performing ultraviolet illumination cross-linking to prepare a first-layer PEG-DMA hydrogel; (3) taking the PEG-DMA, adding into the deionized water to uniformly stir to prepare a second-layer PEG-DMA solution; (4) adding the evocating agent into the second-layer PEG-DMA solution prepared in the step (3), uniformly stirring while keeping out of the sun, feeding into the mold filled with the first-layer PEG-DMA hydrogel, and performing ultraviolet illumination cross-linking to prepare a double-layer PEG-DMA hydrogel; (5) repeating the steps (3) and (4) to prepare the multilayer PEG-DMA hydrogel. By adopting the preparation method provided by the invention, a stable multilayer PEG-DMA hydrogel structure with anisotropism can be constructed.

Description

Technical field [0001] The invention relates to a method for preparing a PEG-DMA hydrogel, in particular to a method for preparing a multilayer PEG-DMA hydrogel. Background technique [0002] In recent years, biomaterials have been widely used in various fields. As a typical biomaterial, hydrogel has good biocompatibility, water content, flexibility, degradability, ease of preparation, Excellent performance such as sensitivity to external stimuli has been widely used in cell culture, tissue engineering, biosensors and actuators, drug delivery systems and other fields. PEG-DMA (polyethylene glycol dimethacrylic acid) is a hydrogel with PEG (polyethylene glycol) macromonomers, due to its good biocompatibility, non-toxicity, non-volatile, and stable Good properties, mechanical properties (modulus of elasticity, Poisson’s ratio) close to parts of human tissues (such as skin, muscle tissue) and modifiable advantages make it popular and widely used in tissue engineering and medicine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B32B27/06B32B27/16B32B27/28C08J3/28C08J3/24
Inventor 徐峰何翔王琳王鹏飞黄国友卢天健
Owner GUANGDONG XIAN JIAOTONG UNIV ACADEMY
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