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Subculture medium for saline/alkaline-tolerant fast-growing ulmus pumila tissue culture seedling
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A technology of subculture medium and tissue culture seedlings, which is applied in plant regeneration, plant cells, horticulture, etc., can solve the problems of low value-added rate of medium, high cost, waste of resources, etc., and achieve high average proliferation rate and growth Vigorous, the effect of reducing production costs
Inactive Publication Date: 2015-03-04
NORTHWEST A & F UNIV +1
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The value-added rate of the above-mentioned culture medium is low, which causes high cost and waste of resources in the factory production of Baiyu
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Embodiment 1
[0017] Pretreatment of the tissue-cultured seedlings of Ulmus ulmis: select the tissue-cultured seedlings of Ulmus ulmis with the same growth cycle, culture conditions, and health, and cut the tissue-cultured seedlings of Ulmus ulmis into multiple single buds and clustered buds of the same size for later use.
[0018] The growth environment required for the tissue cultured seedlings of Ulmus ulmiformis is: temperature 25°C (± 2°C), 12 hours of light time, subculture and culture for 21 days in a sterile environment every day.
[0019] The invention mainly uses basic medium for comparison and screening, adds low-concentration proliferative hormone 6-BA to ensure the normal growth of Ulmus elm tissue culture seedlings, and agar 7g / L and white sugar 30g / L as auxiliary materials.
[0020] In order to compare the difference between the existing white elm production medium and the present invention, the present invention selects ms+BA0.2+S3% (A1), EM+6-BA0.2+S3% (A2), dkw+6-BA0. 2+S3...
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Abstract
The invention discloses a subculture medium for a saline / alkaline-tolerant fast-growing ulmus pumila L. tissue culture seedling. The subculture medium comprises the following components: CaCl2.2H2O, NH4NO3, KNO3, NaCl, MgSO4, NaH2PO4.2H2O, K2SO4, Na2SO4, CaCl2, CoCl2.6H2O, CuSO4.5H2O, H3BO3, MnSO4.H2O, Na2MoO4.2H2O, ZnSO4.7H2O, KI, FeSO4.7H2O, Na2EDTA.2H2O, inositol, glycine, nicotinic acid, V6, V1, biotin, folic acid, riboflavin and vitamin. Compared with a traditional culture medium, the subculture medium disclosed by the invention improves the average reproduction rate of the ulmus pumila L. tissue culture seedling by more than 5 times, and improves the average reproduction coefficient by more than 2.3 times.
Description
technical field [0001] The invention relates to a basic culture medium for tissue-cultured seedlings, in particular to a series of basic culture media for the subculture of saline-alkali-tolerant fast-growing Ulmus ulmis tissue-cultured seedlings. Background technique [0002] White elm (Ulmus pumila L.), a plant of the genus Ulmus in the family Ulmaceae, has many varieties, and elm has always been the top five native garden tree species in northern my country [Populus, Salix, elm (Ulmus), Chinese pagoda tree (Sophora), Ailanthus (Ailanthus)], and one of the world's four major street greening tree species, with a wide range of applications. [0003] White elm is a native tree species with a long history of cultivation in my country. It is mostly used for afforestation in saline-alkali land and arid and barren areas because of its characteristics of drought resistance, high temperature resistance, severe cold resistance, fast growth, good material, resistance to barrenness, an...
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