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Detoxification method of Jatropha curcas cake by fermentation

A technology of jatropha curcas cake and seeds, which is applied in the field of fermentation, can solve the problems that detoxification and nutrition improvement of jatropha curcas cake cannot be taken into account, and achieve the effects of simple method, reduction of toxic components, and low cost

Inactive Publication Date: 2015-02-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current fermentation methods can only reduce the toxicity of Jatropha curcas cake or improve its nutritional value, and cannot take into account the detoxification and nutritional improvement of Jatropha curcas cake

Method used

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  • Detoxification method of Jatropha curcas cake by fermentation
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  • Detoxification method of Jatropha curcas cake by fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 The identification characteristics of Enterobacter japonica and Morganella morganii of the present invention

[0035] 1. Enterobacter diarrhoea

[0036] (1) Physiological and biochemical characteristics

[0037] After the isolated bacterial strains of the present invention were cultured on NB and crystal violet plates for 24 hours, a single colony on the NB plate was taken for Gram staining observation, combined with the growth of the bacterial strain on the crystal violet plate to determine its Gram-negative and positive, and oxidase Experiment and trisaccharide iron experiment, the results are shown in Table 1:

[0038] Table 1 Physiological and biochemical characteristics

[0039]

[0040] From the results of physiological and biochemical experiments, it can be seen that the zxy-15 isolate of the present invention is a Gram-negative bacterium and belongs to intestinal bacteria (GN-ENT). GN identification plate.

[0041] (2) Biolog analysis results...

Embodiment 2

[0065] Embodiment 2 adopts bacterial strain of the present invention to the method for Jatropha curcas cake fermentation detoxification

[0066] 1. Experimental materials

[0067] Bacterial species: Enterobacter gergoviae zxy-15 (Enterobacter gergoviae zxy-15) of the present invention, referred to as strain zxy-15; Morganella morganii zxy-12-4 (Morganella morganii zxy-12-4) of the present invention, referred to as strain zxy-12-4.

[0068] Jatropha cake, including the following 3 types:

[0069] Undeoiled cake: obtained by crushing the dried Jatropha curcas seeds through a 40-mesh sieve.

[0070] Cake after Soxhlet deoiling: crush the dried Jatropha curcas seeds through a 40-mesh sieve, and extract the residue with petroleum ether at 30-60°C for 8 hours in a Soxhlet extractor, which is the Jatropha curcas cake.

[0071] Cake after oil extraction: crush the dried Jatropha curcas seeds through a 40-mesh sieve, extract the oil, and obtain the residue as Jatropha curcas cake. ...

Embodiment 3

[0076] Embodiment 3 adopts bacterial strain of the present invention to the method for Jatropha curcas cake fermentation detoxification

[0077] 1. Experimental materials

[0078] Bacterial species: Enterobacter gergoviae zxy-15 (Enterobacter gergoviae zxy-15) of the present invention, referred to as strain zxy-15; Morganella morganii zxy-12-4 (Morganella morganii zxy-12-4) of the present invention, referred to as strain zxy-12-4.

[0079] Jatropha cake, including the following 3 types:

[0080] Undeoiled cake: obtained by crushing the dried Jatropha curcas seeds through a 40-mesh sieve.

[0081] Cake after Soxhlet deoiling: crush the dried Jatropha curcas seeds through a 40-mesh sieve, and extract the residue with petroleum ether at 30-60°C for 8 hours in a Soxhlet extractor, which is the Jatropha curcas cake.

[0082] Cake after oil extraction: crush the dried Jatropha curcas seeds through a 40-mesh sieve, extract the oil, and obtain the residue as Jatropha curcas cake. ...

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Abstract

The invention discloses a detoxification method of Jatropha curcas cake by fermentation, which comprises the following steps: (1) fetching Enterobacter gergoviae and Morganella morganii, respectively recovering and preparing Enterobacter gergoviae seed liquid and Morganella morganii seed liquid, and mixing the seed liquids according to a ratio 1:2-2:1 (v / v), and obtaining a seed liquid; (2) fetching a Jatropha curcas cake, adding water and carbon source, mixing well, inoculating the seed liquid prepared in the step (1), wherein, the ratio between the material and the water is 1:0.8-1.2 (w / v), and the addition of the carbon source is 10-20% (w / w) of the Jatropha curcas cake. The inoculation amount of the seed liquid is 10-30% (v / w) of the Jatropha curcas cake; (3) the inoculated cake is fermented for 2-8 days at 30-37 DEG C. The invention can effectively reduce toxicity of the Jatropha curcas cake, and simultaneously improve the nutrition value with good market application prospect.

Description

technical field [0001] The invention relates to a fermentation and detoxification method of Jatropha curcas cake, which belongs to the field of fermentation. Background technique [0002] Jatropha curcas (Jatropha curcas L.) is recognized worldwide as the most likely tree species with great development potential to replace fossil energy in the future, and the biodiesel produced by processing Jatropha curcas oil is close to or even surpasses the national " No. 0" diesel oil and national biodiesel standards, so the production of biodiesel from Jatropha curcas fruit has become an active direction of research and production of biodiesel. Jatropha curcas seed cake (JCSC) is a by-product of the biodiesel production process. It has a high protein content, but it is difficult to utilize the protein, and it is highly toxic. It is mostly used as fertilizer or directly Discharging into the natural environment not only seriously wastes a large amount of high-quality protein resources, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/015A23L5/20
CPCA23L5/28A23L29/065
Inventor 陈放张晓喻唐琳尹利刘莹
Owner SICHUAN UNIV
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