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Method for preparing chitosan by xylose in hemicellulose hydrolysate

A technology of hemicellulose and hydrolyzate, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of undiscovered patent publications, etc., and achieve the effects of simple method, reduced environmental pollution, and convenient operation.

Inactive Publication Date: 2015-02-18
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • Method for preparing chitosan by xylose in hemicellulose hydrolysate

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Embodiment 1

[0033] A method utilizing xylose in hemicellulose hydrolyzate to prepare chitosan, the steps are as follows:

[0034] ⑴Hydrolysis of hemicellulose raw materials: After the corn cob is crushed, it is sieved so that the particle size does not exceed 1mm; the crushed corn cob is mixed with H 2 SO 4 Solution by cob: H 2 SO 4 The solid-to-liquid ratio g:mL of the solution is 1:10, and hydrolyzed in a hydrolysis pot at 120°C for 2 hours to obtain a hemicellulose hydrolyzate;

[0035] (2) Detoxification and decolorization treatment: add Ca(OH) to the hemicellulose hydrolyzate obtained in step (1) 2 Adjust the pH to 10, and obtain a supernatant after solid-liquid separation, and use 10% H in the supernatant 2 SO 4 The pH of the solution is adjusted to 7, and the solid-liquid separation obtains the supernatant; 2 Add activated carbon to the detoxified supernatant at a solid-to-liquid ratio of 1:20 (g:mL), incubate at 50°C for 1 hour, and filter to obtain the detoxified and decolo...

Embodiment 2

[0048] A method utilizing xylose in hemicellulose hydrolyzate to prepare chitosan, the steps are as follows:

[0049] ⑴Hydrolysis of hemicellulose raw materials: After the corn cob is crushed, it is sieved so that the particle size does not exceed 1mm; the crushed corn cob is mixed with H 2 SO 4 Solution by cob: H 2 SO 4 The solid-to-liquid ratio g:mL of the solution is 1:10, and hydrolyzed in a hydrolysis pot at 100°C for 2 hours to obtain a hemicellulose hydrolyzate;

[0050] (2) Detoxification and decolorization treatment: add Ca(OH) to the hemicellulose hydrolyzate obtained in step (1) 2 Adjust the pH to 9, obtain the supernatant after solid-liquid separation, and use 10% H in the supernatant 3 PO 4 The pH of the solution is adjusted to 6, and the solid-liquid separation obtains the supernatant; 2 Add activated carbon to the supernatant after detoxification treatment at a solid-to-liquid ratio of 1:50 (g:mL), incubate at 50°C for 0.5h, filter to obtain hemicellulose ...

Embodiment 3

[0062] A method utilizing xylose in hemicellulose hydrolyzate to prepare chitosan, the steps are as follows:

[0063] ⑴Hydrolysis of hemicellulose raw materials: After the corn cob is crushed, it is sieved so that the particle size does not exceed 1mm; the crushed corn cob is mixed with H 2 SO 4 Solution by cob: H 2 SO 4 The solid-to-liquid ratio g:mL of the solution is 1:10, and hydrolyzed in a hydrolysis pot at 120°C for 3 hours to obtain a hemicellulose hydrolyzate;

[0064] (2) Detoxification and decolorization treatment: add Ca(OH) to the hemicellulose hydrolyzate obtained in step (1) 2 Adjust the pH to 11, obtain the supernatant after solid-liquid separation, and use 10% H in the supernatant 2 SO 4 The pH of the solution is adjusted to 8, and the solid-liquid separation obtains the supernatant; 2 Add activated carbon to the supernatant after detoxification treatment at a solid-to-liquid ratio of 1:25 (g:mL), incubate at 50°C for 2 hours, and filter to obtain hemice...

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Abstract

The invention relates to a method for preparing chitosan by xylose in hemicellulose hydrolysate. The method comprises the following steps of: (1) after smashing a lignocellulose material and performing acid hydrolysis for the hemicellulose; (2) detoxifying and decoloring the hemicellulose hydrolysate; (3) culturing filamentous fungi by using detoxified hemicellulose hydrolysate as a carbon source; (4) removing binding proteins of the filamentous fungi by an alkali liquor; and (5) extracting chitosan from filamentous fungi by a weak acid. According to the method provided by the invention, the hemicellulose hydrolysate is detoxified and decolored by Ca(OH)2 and active carbon, and poisonous components (such as formic acid, formic acid and furfural) in the treated hemicellulose hydrolysate are remarkably reduced, so that a good growing environment is provided for mycelium. Therefore, hemicellulose in lignocellulose is utilized and converted, and the yield of chitosan is improved. The method is simple and convenient to operate.

Description

technical field [0001] The invention relates to the technical field of biomass utilization, in particular to a method for preparing chitosan by using xylose in hemicellulose hydrolyzate. Background technique [0002] Chitosan is the only natural alkaline polysaccharide discovered so far that exists in large quantities, and has unique properties and physiological functions, such as permeability, fibrillation, hygroscopicity, viscosity-increasing, film-forming, adsorption, and antibacterial properties , biocompatibility, moisture retention, etc. In recent years, it has been widely used in the fields of environmental protection, textile printing and dyeing, food, chemical industry, cosmetics, water treatment and biomedicine. At present, the production method of chitosan is mainly processed and extracted from shrimp and crab shells with acid and alkali. This process needs to consume a large amount of acid and alkali, is highly corrosive, and has a large amount of waste liquid, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12R1/645
Inventor 赵华王希
Owner TIANJIN UNIV OF SCI & TECH
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