Production method and application for organic yeast protein selenium product with life combined state

A yeast protein, combined state technology, applied in the application, animal feed, fungi and other directions, can solve the problems of no complex method, little yeast selenium production, low tolerance of yeast to inorganic selenium, etc. The effect of substrate loss and energy consumption, improving raw material utilization, strong low toxicity and high efficiency

Inactive Publication Date: 2014-04-30
广州市博仕奥生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent ZL201210496430.8 provides a simple mixing method to prepare selenium-enriched biological bacterial fertilizer, without converting inorganic selenium into organic selenium, and there is no specific complexing method
[0005] However, yeast selenium products rarely achieve significant effects during application; in addition, yeast selenium is rarely produced in large quantities
The main problems: (1) Selenium content in yeast selenium products is low; (2) yeast has low tolerance to inorganic selenium; (3) organic selenium content in yeast selenium is low, part of which is simply yeast and selenium adsorption; (4) The utilization rate of medium raw materials is low, the cost is high, and the production process is complicated, and the repeatability is poor; (5) The absorption rate, safety and bioavailability of yeast selenium products are low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The production process is as follows: slant strain cultivation→first-level liquid seed cultivation→secondary liquid seed cultivation→fermentation tank seed cultivation→fermentation tank cultivation→separation→drying→packaging.

[0029] (1) Incline strain culture: Streak yeast on peptone 5g / L, yeast extract 15g / L, sodium chloride 4g / L, glucose 10g / L, nano-selenium 120mg / L, agar 20g / L, pH value It is activated on a 4.8-6.0 solid plate, cultivated at a constant temperature of 31°C for 48 hours, and picks a single colony slant and saves it for later use.

[0030] (2) First-level liquid seed culture: Pick 1 to 4 rings of yeast strains on the slope and inoculate them in a container containing 100 to 500 mL of peptone 5g / L, yeast extract 15g / L, sodium chloride 4g / L, and glucose 10g / L. , nano-selenium 120mg / L, pH value is 4.8~6.0 in the Erlenmeyer flask of liquid culture medium, at 31 ℃ constant temperature culture for 48 hours.

[0031] (3) Secondary liquid seed culture: Inoc...

Embodiment 2

[0039] The production process is as follows: slant strain cultivation→first-level liquid seed cultivation→secondary liquid seed cultivation→fermentation tank seed cultivation→fermentation tank cultivation→separation→drying→packaging.

[0040] (1) Slant strain culture: Streak yeast on peptone 5g / L, yeast extract 15g / L, sodium chloride 4g / L, glucose 10g / L, nano-selenium 150mg / L, agar 20g / L, pH value It is activated on a 4.8-6.0 solid plate, cultivated at a constant temperature of 31°C for 48 hours, and picks a single colony slant and saves it for later use.

[0041](2) First-level liquid seed culture: Pick 1 to 4 rings of yeast strains on the slope and inoculate them in a container containing 100 to 500 mL of peptone 5g / L, yeast extract 15g / L, sodium chloride 4g / L, and glucose 10g / L. , nano-selenium 150mg / L, and the pH value is 4.8~6.0 in the Erlenmeyer flask of liquid culture medium, at 31 ℃ constant temperature culture 48 hours.

[0042] (3) Secondary liquid seed culture: Ino...

Embodiment 3

[0077] Produce 25 tons of fattening pig feed in the feed factory, the amount of the yeast selenium product added in the diet is 40mg / kg, 180 Dudu big fattening pigs with a body weight of about 65kg are divided into 6 groups according to the feeding requirements, and each group has 3 repetitions. Each replicated 10 heads and carried out a 45-day breeding experiment.

[0078] Table 1 Quality table of fattening pork

[0079] index control group common yeast selenium group Comparative example 1 Comparative example 2 Example 1 product group Example 2 product group PH1 6.01 6.11 6.17 6.19 6.40 6.23 PH24 5.47 5.50 5.62 5.64 5.85 5.69 Drip loss (%) 6.57 5.01 3.81 3.56 2.06 2.02 Selenium deposition (mg / kg) 0.056 0.209 0.306 0.322 0.460 0.481

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Abstract

The invention relates to a production method and an application for an organic yeast protein selenium product with a life combined state. According to the production method and the application disclosed by the invention, a selenium source used during a fermentation process is nano-selenium; nano-selenium has the advantages of being low in toxicity, large in specific surface area, high in bioactivity, and the like, and is capable of increasing the tolerance dose of yeasts to selenium and the bioavailability; protease is added in a yeast fermentation culture medium to increase the utilization rate of fermentation materials and reduce cost. Compared with the previous method of converting inorganic selenium to organic selenium by yeasts, the selenium source used in the production method and the application disclosed by the invention is nano-selenium, and has the advantages of being low in toxicity, high in conversion rate, high in absorption rate, good in product safety performance, high in bioactivity, convenient for large-scale production and collection, and the like.

Description

technical field [0001] The invention relates to a production method and application field of a yeast protein selenium product, in particular to a production method and application of a life-bound organic yeast protein selenium product. Background technique [0002] Selenium is an essential trace element for humans and animals, and an important component of various enzymes in the body. It has the functions of anti-oxidation, regulating body metabolism, and enhancing immunity. However, the safety limit of selenium between the optimal concentration and the toxic concentration is very narrow, so the development of low-toxicity and high-efficiency selenium sources has always been the focus of selenium nutrition research. Nano-selenium is a new type of selenium product, which has the characteristics of low toxicity and high bioavailability. Announcement of the Ministry of Agriculture of the People's Republic of China (No. 1226), Catalog of Feed Additives (2008), there is no nano-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K1/16A23K1/18
Inventor 刘东霞杜景德
Owner 广州市博仕奥生物科技有限公司
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