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Layered-type cell culture plate

A cell culture and culture plate technology, applied in the field of medical and laboratory equipment, can solve the problems of high price and increase in cost, and achieve the effect of simple production, low cost and obvious effect.

Inactive Publication Date: 2014-05-07
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, in the process of cytological research, it is often necessary to culture cells to meet the specific requirements of various experiments. However, in the process of cell culture, because of the need for sterilization, the conventional method is to use various disposable Consumables are used for cell biology experiments. The price of each consumable is relatively high. For example, a six-well plate costs 7 yuan. After one experiment, multiple six-well plates are needed, which doubles the cost of the experiment. to grow

Method used

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  • Layered-type cell culture plate
  • Layered-type cell culture plate
  • Layered-type cell culture plate

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Embodiment 1

[0023] Layered cell culture plate consists of culture plate 1, culture plate hole 2, culture plate groove 3, culture plate groove wall 4, microporous filter membrane 5, plate hole column 6, culture plate axis hole 7, culture hole 8, culture plate 9. The culture plate hole wall 10, the culture plate hole cavity 11, the support column 12, the culture hole wall 13, the Teflon layer 14, the culture hole opening 15, the culture hole bottom 16, and the culture hole substrate 17 are characterized in that the culture plate 1 is made of glass, and the upper part has a culture plate groove 3 with a depth of 5-10 mm inward. In the culture plate groove 3, there are 6 culture plate holes 2; the upper part of the culture plate hole 2 is higher than the culture plate groove 3 bottom, so that the microporous filter membrane 5 can wrap the outside of the opening of the culture plate hole 2 and seal the opening of the culture plate hole 2 after winding; there is a plate hole column 6 in the cent...

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Abstract

The invention provides a layered-type cell culture plate and belongs to the technical field of medical apparatus and instruments. The layered-type cell culture plate is composed of a culture plate, culture plate holes, a culture plate trough, culture plate trough walls, microfiltration membranes, plate hole stand pillars, cultivation plate shaft holes, cultivation holes, cultivation plates, culture plate hole walls, culture plate hole cavities, support pillars, cultivation hole walls, Teflon layers, cultivation hole openings, cultivation hole bottoms and cultivation hole substrates. The layered-type cell culture plate is characterized in that, the culture plate is made of glass; a culture plate trough is arranged in the upper part inward; there are six culture plate holes in the culture plate trough; the upper half part of each culture plate hole is higher than the bottom of the culture plate trough; a plate hole stand pillar is arranged in the center of each culture plate hole, the plate hole stand pillar goes through cultivation plate shaft holes and support pillars, and cultivation plates can be overlaid together layer by layer; the support pillars are glass cylinders separating the cultivation plates by 2mm; a plurality of cultivation holes are arranged in the upper surface of each cultivation plate regularly. The layered-type cell culture plate is advantaged by simple manufacturing, high operability, low cost and obvious effects.

Description

technical field [0001] The invention relates to a layered cell culture plate, which belongs to the technical field of medical treatment and laboratory equipment. Background technique [0002] At present, in the process of cytological research, it is often necessary to culture cells to meet the specific requirements of various experiments. However, in the process of cell culture, because of the need for sterilization, the conventional method is to use various disposable Consumables are used for cell biology experiments. The price of each consumable is relatively high. For example, a six-well plate costs 7 yuan. After one experiment, multiple six-well plates are needed, which doubles the cost of the experiment. growing. In the process of cell culture, one of the signs of whether the cells are successfully cultured is whether the cells adhere to the wall, and whether they divide and multiply after adherence. Thus, a larger support area for cell growth is required for better r...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M23/12C12M23/20C12M23/42C12M37/02
Inventor 张云华陈丽娟陈迅何金铃张宽朝魏晓飞王宝玉
Owner ANHUI AGRICULTURAL UNIVERSITY
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