Method for detecting ractopamine content in food

A technology of ractopamine and detection method, which is applied in the direction of testing food, biological testing, material inspection products, etc., to achieve the effect of strong identification and enrichment function, low price, and improved solubility and stability

Active Publication Date: 2015-06-10
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing ractopamine is no longer suitable for this shift in detection direction

Method used

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  • Method for detecting ractopamine content in food
  • Method for detecting ractopamine content in food
  • Method for detecting ractopamine content in food

Examples

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Embodiment 1

[0041] Detection of ractopamine content in pork

[0042] Different pork and liver samples were purchased from local supermarkets and processed separately according to conventional methods. The processing method is briefly described as follows:

[0043] After the sample was minced, 1.0 g of it was uniformly dispersed in 2 mL of 3% trichloroacetic acid, vortexed until uniform, and then 1 mL of acetonitrile was added and shaken well. Subsequently, the supernatant was collected by centrifugation at 4000 rpm for 10 minutes. After that, the collected supernatant was adjusted to pH 9.8±0.2 with 10 wt% sodium carbonate, and then 0.8 g of sodium chloride was added. Afterwards, the mixture was extracted twice with 2 mL of ethyl acetate, and centrifuged at 4000 rpm for 10 minutes. Take the upper layer liquid and blow dry under nitrogen flow at 50-60°C, add 1 ml of PBS buffer and shake well for detection.

[0044] A total of ten pork samples were prepared in this example, and ten pig ...

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Abstract

The invention relates to a method for detecting ractopamine content in food. The method is characterized by comprising the following steps: preparing a capture probe; preparing a signal probe, treating a sample to be detected, and detecting by using a glucometer. Compared with the prior art, the method has the advantages that the signal is converted into a glucose signal which can be detected by the glucometer through an enzymatic effect, field detection of a nonsugar glucometer is realized, the stability is high, the detection limit is low, the detection range is wide. Moreover, the detection cost is low, the detection program is simple, the detection effect is good, particularly general staff can perform field detection, and the strict requirement that the professional staff need to operate in a lab in the prior art is avoided.

Description

technical field [0001] The invention relates to a method for detecting and analyzing substances, in particular to a method for detecting the content of ractopamine in food. Background technique [0002] Ractopamine is a β-stimulant, which can transfer nutrients in animals from fat to muscle, showing a nutrient redistribution effect, thereby regulating the material metabolism of animals, enhancing fat decomposition, promoting protein synthesis, and significantly improving carcass lean meat rate and feed remuneration. However, their abuse and residues in animal food seriously endanger people's health and life safety, and seriously affect the export trade of my country's livestock and poultry products. Most countries in the world have expressly banned the use of ractopamine as a feed additive. [0003] For the detection of ractopamine, relatively mature methods and technologies are currently used, such as mass spectrometry, chromatography, spectroscopy and enzyme-linked immun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/02G01N33/53
Inventor 干宁陈思曹玉廷胡富陶李天华崔焕张佳斌王德熊萍
Owner NINGBO UNIV
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