Single particle or single molecule tracking device and method based on atomic force microscope

Abstract

The invention relates to a single particle or single molecule tracking device and method based on an atomic force microscope. The tracking method comprises the following steps that before tracking measurement of a single particle or a single molecule is conducted, the point of a probe of the atomic force microscope is trimmed with a detection object; in the process of tracking measurement, the probe of the atomic force microscope makes contact with the surface of a detected object with constant force; when the detection object and the detected object act to each other, a micro cantilever deflects, and the telescopic distance of piezoelectric ceramics of a scanner is changed; a data acquisition card is used for collecting the changes over time of the deflection of the micro cantilever and the changes over time of the telescopic distance of the piezoelectric ceramics, so that the single particle or the single molecule is tracked. The single particle or single molecule tracking method based on the atomic force microscope can be applied to a study on the dynamic process of cell endocytosis viruses, nano particles and other particles, and is also suitable for a study on the transfer process of glucose, amino acid and other small biological molecules on cytomembrane.

Description

technical field [0001] The invention relates to the technical field of microscopic particle track measurement methods, in particular to an atomic force microscope-based single-particle or single-molecule tracing device and tracing method. Background technique [0002] The study of the interaction mechanism between biomolecules at the single-molecule level is of great significance for the in-depth understanding of the specific recognition of biomolecules, biochemical processes, and the relationship between molecular structure and function, and has become an important research field in the interdisciplinary fields of biology, chemistry, and physics. A Frontier Direction ([1] Yizhang Chen, Qisho Lin, Single Molecule Behavior and Intracellular Real-Time Detection in Life Sciences, 1, Beijing: Science Press, 2005, 1-11. [2] M.J.J.A.Dvorak, The application of atomic force microscopy to the study of living vertebral cells in culture, Methods, 2003(29): 86-96). [0003] With its un...

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Problems solved by technology

[0005] The above-mentioned AFM single-molecule force spectroscopy method has certain defects in the study of the interaction between single particles and cells. During the experiment, the AFM probe is driven by the piezoelectric ceramic scanner to move toward the cell surface in the Z direction, and the cell surface Dynamic processes on the surface, such as endocytosis, transport, etc. are also movements in the Z direction, such as figure 1 As shown, this makes it easy to consider the detected force signal to be generated by the movement of the AFM probe itself rather than the dynamic process on the cell surface, so it is impossible to directly and objectively study the dynamic process on the cell surface

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